78764

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 128614..130182 Replicon plasmid pA18-1
Accession NZ_CP022274
Organism Citrobacter freundii strain 18-1

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag CFA70_RS27425 Protein ID WP_024134513.1
Coordinates 128614..129921 (-) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag CFA70_RS27430 Protein ID WP_039272366.1
Coordinates 129931..130182 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFA70_RS27395 123758..124276 - 519 WP_079778878.1 hypothetical protein -
CFA70_RS27400 124496..124798 + 303 WP_001161555.1 hypothetical protein -
CFA70_RS27410 125170..126810 - 1641 WP_088903423.1 PAAR domain-containing protein -
CFA70_RS27415 127009..127947 + 939 WP_000395773.1 hypothetical protein -
CFA70_RS27420 128123..128617 - 495 WP_039272364.1 hypothetical protein -
CFA70_RS27425 128614..129921 - 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
CFA70_RS27430 129931..130182 - 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
CFA70_RS27435 130545..131549 - 1005 WP_001553091.1 tyrosine-type recombinase/integrase -
CFA70_RS27440 131552..132115 - 564 WP_001132404.1 hypothetical protein -
CFA70_RS27445 132134..132415 - 282 WP_000575656.1 hypothetical protein -
CFA70_RS27450 132676..132960 + 285 Protein_161 hypothetical protein -
CFA70_RS27455 133151..133399 - 249 Protein_162 chemotaxis protein -
CFA70_RS27460 133678..134682 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..159655 159655
- inside IScluster/Tn - - 133678..146773 13095


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T78764 WP_024134513.1 NZ_CP022274:c129921-128614 [Citrobacter freundii]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

>T78764 NZ_CP022274:c129921-128614 [Citrobacter freundii]
ATGCAGACGCTAACAGTGGCGATGAATGGTGAGGTCGTAGGAACACTGTACCGAGATAACAAAGGGGCCATGTCATTTCA
GTATGCCCCGGAGTGGATCTCGGAACCAGGTTCACGCGCCATTTCCATGTCACTGCCTTTGGGTCATGGCAGGATCACAG
GAAGTGAAGTCTTCAATTTCTTTAGCAACCTCCTACCCGACTCAGAGGCAATTATTGCCAGGATGCAGGCTAGGTTCAGA
GTTGAGACAACACATCCATTTGATTTGCTGGCGAGTGTTGGCCGGGATTGTGTTGGAGCTATCCAGCTCTATTCCCCCGA
TGCGGACATCCCAACTGTAATGGAGACGCTTGCAGAGCCTCTGGATGAAGCTCAGATTGAGCAACTGCTTGAAGGCTACC
AGGAAGCGCCCCTTGGGATGGCAGAGGATGCTGATTTTCGGATCTCGATTGCCGGAGCCCAAGAGAAAACTGCACTGCTT
TGGTATCAGGACCGCTGGCAACGCCCCCTGGGCAGCACCCCAACAAGCCATATTTTTAAGCTACCCATTGGTAAGATTGT
TCAAAATAACATCGATCTCAGTGAGAGTTGTGAGAATGAATGGCTCTGTTTGCGCATTGCAAAGGCCTTCGGTTTTGACG
TCGCCAATGCGGACCTAGCAACGTTCGGCAAGAAGAAGGTGCTTGTCGTTCAGCGTTTTGACCGGCGCTGGTCTAAATCC
GGCGAATGGCTTCTACGATTGCCCCAAGAAGACTTCTGCCAAGCTCTTGGGATCGCTCCTGCGCTCAAATACGAGTCACA
TGGAGGGCCAGGTATTGCTGACGCAATGAAGTTGTTGTTGGGGTCCAGACTTGCGGCTAAGGATAGAGAGCAATTTTTCA
AGTCGCAGATCCTATTCTGGATGTTGGCCGCGATTGATGGGCACGGGAAGAACTTCTCCATCTTCATTGAGCCTGCATCG
TCATTTAGGATGACCCCCCTCTACGATGTCATGTCTGCTTTTCCTATCTTCAAAAGCGGGGGAATTGCTGCTCAAAAGGC
AAAGATGGGGATGGCGCTCCAAGGCAAAAACCGCCAATACCATTTTGCGATGATCCAGCCTCGTCATTTTATCAGTACCG
CCGCCCATGTCGGGTTCCCACAAGATACCGCCGCTCAACTTATGTTCGACATGGCTACCAAGACAGAGGAGGTGGTTGCC
ACAGTAGCGGCTGAGCTGCCAGCCGGTTTCCCAGAGCACATCAGTAGCGCCATCTTCGAGGGGCTGTCCAGCCAGGCGGC
TAAGATCTTGAAGGGAGTAGCCAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT78764 WP_039272366.1 NZ_CP022274:c130182-129931 [Citrobacter freundii]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp

>AT78764 NZ_CP022274:c130182-129931 [Citrobacter freundii]
ATGAAAGTGACATCTCCATCCTTGCTTGCGGCTGCGGTGCGTGACCAGCGCAAGCTCAGCAAACTAACGCAGTCAGAGGC
GGCGAAACAGGTCGGAATCAAGCAGACGACCGTCTCTGATTTTGAACTACGGCCAGAATCTACCAAGCTGGAGACGCTAT
TCAAGCTCCTTTCTGCACTGGATCTGGAGCTTCATGTCGTGAAGCGGGGTTCAACATTGGACGATAGTAAAGTCTGGGAT
AGGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References