Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/ParE-RHH
Location 76858..77377 Replicon plasmid pA18-1
Accession NZ_CP022274
Organism Citrobacter freundii strain 18-1

Toxin (Protein)


Gene name parE Uniprot ID A0A837F4A9
Locus tag CFA70_RS27065 Protein ID WP_000277490.1
Coordinates 77093..77377 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A6P1V8C3
Locus tag CFA70_RS27060 Protein ID WP_000124732.1
Coordinates 76858..77100 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFA70_RS27040 72930..73904 - 975 Protein_92 DNA-binding protein -
CFA70_RS27045 73940..75874 - 1935 Protein_93 Tn3 family transposase -
CFA70_RS27050 75936..76640 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
CFA70_RS27060 76858..77100 + 243 WP_000124732.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
CFA70_RS27065 77093..77377 + 285 WP_000277490.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CFA70_RS27070 77515..77607 - 93 Protein_97 DNA partition complex ParG -
CFA70_RS27075 77929..78951 - 1023 WP_001567660.1 IS110 family transposase -
CFA70_RS27080 78975..79772 - 798 Protein_99 Tn3 family transposase -
CFA70_RS27090 79837..80541 + 705 WP_088903415.1 IS6-like element IS26 family transposase -
CFA70_RS27095 80701..81387 + 687 WP_041205587.1 hypothetical protein -
CFA70_RS27100 81384..81845 + 462 WP_005326786.1 YjiG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..159655 159655
- inside IScluster/Tn - - 73940..88100 14160


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-88)

Antitoxin

Antitoxin ParD

(1-64)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11005.52 Da        Isoelectric Point: 6.2103

>T78763 WP_000277490.1 NZ_CP022274:77093-77377 [Citrobacter freundii]
VYKLTERAADDFAGIYDYTLLKFGEAQADHYTDALEAFFETLAGMPDMGRDYHTVPGVMRIEFQRHAIFYTVRDTDILIA
RILHQQMNHKRHLL

Download         Length: 285 bp

>T78763 NZ_CP022274:77093-77377 [Citrobacter freundii]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8875.01 Da        Isoelectric Point: 7.0130

>AT78763 WP_000124732.1 NZ_CP022274:76858-77100 [Citrobacter freundii]
MARTTSVTIGEQLDSFISRLIDSGRYGSASEVMRSALRLLEQQETNDEVIRQAVIAGLESGESSLSLRDIAAQRKLRHRV

Download         Length: 243 bp

>AT78763 NZ_CP022274:76858-77100 [Citrobacter freundii]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837F4A9

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V8C3

References