Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7170..7813 Replicon plasmid pA18-1
Accession NZ_CP022274
Organism Citrobacter freundii strain 18-1

Toxin (Protein)


Gene name vagD Uniprot ID A0A5P6KDG5
Locus tag CFA70_RS26565 Protein ID WP_021312768.1
Coordinates 7397..7813 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A5P6KGW8
Locus tag CFA70_RS26560 Protein ID WP_021312767.1
Coordinates 7170..7400 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFA70_RS26540 4079..4629 + 551 Protein_2 recombinase family protein -
CFA70_RS26545 4644..4892 + 249 WP_088903409.1 hypothetical protein -
CFA70_RS26550 6557..6739 + 183 WP_071785599.1 mdtC domain protein -
CFA70_RS26560 7170..7400 + 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CFA70_RS26565 7397..7813 + 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin Toxin
CFA70_RS26570 7911..8144 - 234 WP_089617563.1 helix-turn-helix domain-containing protein -
CFA70_RS26580 8150..8854 - 705 WP_001572363.1 IS6-like element IS26 family transposase -
CFA70_RS26585 8919..10358 + 1440 Protein_9 IS66 family transposase -
CFA70_RS26590 10394..10918 + 525 Protein_10 IS5-like element ISKpn26 family transposase -
CFA70_RS26595 11238..11306 + 69 Protein_11 IS6 family transposase -
CFA70_RS26600 11586..12590 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..159655 159655
- inside IScluster/Tn - - 1212..24007 22795


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15175.60 Da        Isoelectric Point: 7.2452

>T78762 WP_021312768.1 NZ_CP022274:7397-7813 [Citrobacter freundii]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPDLVLEDWVR

Download         Length: 417 bp

>T78762 NZ_CP022274:7397-7813 [Citrobacter freundii]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGTGGCCACCGTATCGTGGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGACCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCATGCCATTGCGGCCGGCGCCGTACTGGTGACAAATAATGTGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8722.81 Da        Isoelectric Point: 4.4692

>AT78762 WP_021312767.1 NZ_CP022274:7170-7400 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT78762 NZ_CP022274:7170-7400 [Citrobacter freundii]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCGCGCAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGAAGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5P6KDG5

Antitoxin

Source ID Structure
AlphaFold DB A0A5P6KGW8

References