Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3891460..3892213 Replicon chromosome
Accession NZ_CP022273
Organism Citrobacter freundii strain 18-1

Toxin (Protein)


Gene name yeeV Uniprot ID A0A097QYR4
Locus tag CFA70_RS20020 Protein ID WP_025799003.1
Coordinates 3891836..3892213 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7T0E0C6
Locus tag CFA70_RS20015 Protein ID WP_072269511.1
Coordinates 3891460..3891786 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFA70_RS19995 3886553..3890023 + 3471 WP_088903055.1 AIDA repeat-containing protein -
CFA70_RS20000 3890246..3890677 + 432 WP_088903392.1 antirestriction protein -
CFA70_RS20005 3890689..3891168 + 480 WP_025798999.1 DNA repair protein RadC -
CFA70_RS20010 3891182..3891403 + 222 WP_045888217.1 DUF987 domain-containing protein -
CFA70_RS20015 3891460..3891786 + 327 WP_072269511.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
CFA70_RS20020 3891836..3892213 + 378 WP_025799003.1 TA system toxin CbtA family protein Toxin
CFA70_RS20025 3892210..3892701 + 492 WP_045888216.1 hypothetical protein -
CFA70_RS20030 3892730..3892933 + 204 WP_025799007.1 DUF957 domain-containing protein -
CFA70_RS20035 3893015..3893860 + 846 WP_025799009.1 DUF4942 domain-containing protein -
CFA70_RS20045 3894164..3894670 + 507 WP_048218614.1 G/U mismatch-specific DNA glycosylase -
CFA70_RS20050 3894701..3896698 - 1998 WP_088903056.1 TonB-dependent receptor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(4-103)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13993.97 Da        Isoelectric Point: 6.8515

>T78759 WP_025799003.1 NZ_CP022273:3891836-3892213 [Citrobacter freundii]
MQTQPVPPTQEASPRPSPVEIWLRLLSHLLDRHYGLTLNDTPFCNDGVIQEHIDAGISLCDAVNFIVEKYALIRTDRHGF
SAETQSPLISSIDILRARKACGLMARNGYRLVTDITTGKYSEVTR

Download         Length: 378 bp

>T78759 NZ_CP022273:3891836-3892213 [Citrobacter freundii]
ATGCAAACACAACCTGTTCCCCCGACACAGGAGGCTTCACCACGCCCGTCACCAGTGGAGATCTGGCTCCGCCTTTTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGTTAAACGACACGCCATTTTGCAATGATGGTGTGATCCAGGAGCATATCG
ACGCCGGTATCTCACTGTGCGACGCGGTGAATTTTATCGTGGAGAAATACGCCCTGATACGCACCGATCGTCACGGTTTC
AGCGCAGAAACACAGTCCCCACTCATCAGCAGTATTGATATCCTTCGCGCCCGTAAGGCCTGTGGACTGATGGCACGCAA
TGGCTACAGACTGGTGACCGATATCACCACCGGCAAATACAGCGAGGTGACTCGATGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12016.71 Da        Isoelectric Point: 6.4674

>AT78759 WP_072269511.1 NZ_CP022273:3891460-3891786 [Citrobacter freundii]
IAEPWWGLKRDITPCFGARLVQEGNRLHYLADRASITGQFSDADLRHLDQAFPLLLKQLELMLTSGELNPRHQHGVTLYA
KGLTCNADTLGSCGYVYIAIYPTPATTE

Download         Length: 327 bp

>AT78759 NZ_CP022273:3891460-3891786 [Citrobacter freundii]
ATCGCCGAACCCTGGTGGGGGCTTAAACGCGATATCACACCCTGTTTTGGCGCTCGCCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTCGCCGACCGCGCCAGTATCACCGGGCAGTTCAGCGACGCGGATTTACGCCATCTGGACCAGGCATTTCCGC
TGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCAATCCCCGCCATCAGCACGGCGTCACGCTCTACGCA
AAAGGGCTGACCTGCAACGCGGATACACTCGGCAGTTGCGGATATGTCTATATAGCGATTTACCCGACACCTGCAACAAC
AGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A097QYR4

Antitoxin

Source ID Structure
AlphaFold DB A0A7T0E0C6

References