Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3461770..3462401 Replicon chromosome
Accession NZ_CP022273
Organism Citrobacter freundii strain 18-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A0J1MPQ6
Locus tag CFA70_RS17750 Protein ID WP_003837806.1
Coordinates 3461770..3462045 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0J1MMS0
Locus tag CFA70_RS17755 Protein ID WP_003837808.1
Coordinates 3462042..3462401 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFA70_RS17740 3457821..3460568 + 2748 WP_003846160.1 ribosome-associated ATPase/putative transporter RbbA -
CFA70_RS17745 3460568..3461692 + 1125 WP_003837801.1 ABC transporter permease -
CFA70_RS17750 3461770..3462045 + 276 WP_003837806.1 type II toxin-antitoxin system HicA family toxin Toxin
CFA70_RS17755 3462042..3462401 + 360 WP_003837808.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CFA70_RS17760 3462514..3462915 - 402 WP_003023387.1 nickel-responsive transcriptional regulator NikR -
CFA70_RS17765 3462965..3463543 - 579 WP_003023390.1 4'-phosphopantetheinyl transferase AcpT -
CFA70_RS17770 3463606..3464655 - 1050 WP_003023393.1 AI-2E family transporter -
CFA70_RS17775 3464789..3466005 + 1217 Protein_3326 MFS transporter -
CFA70_RS17780 3466009..3466566 - 558 WP_003837816.1 DcrB family lipoprotein -
CFA70_RS17785 3466639..3467304 - 666 WP_003837818.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10210.88 Da        Isoelectric Point: 10.7228

>T78758 WP_003837806.1 NZ_CP022273:3461770-3462045 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Download         Length: 276 bp

>T78758 NZ_CP022273:3461770-3462045 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAAGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13198.98 Da        Isoelectric Point: 4.5462

>AT78758 WP_003837808.1 NZ_CP022273:3462042-3462401 [Citrobacter freundii]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Download         Length: 360 bp

>AT78758 NZ_CP022273:3462042-3462401 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTTAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MPQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1MMS0

References