Detailed information of TA system TA078757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3007104..3007720
• Replicon: chromosome
• Accession: NZ_CP022273
• Organism: Citrobacter freundii strain 18-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0J1MQ96
• Locus tag: CFA70_RS15475
• Protein ID: WP_003028682.1
• Coordinates: 3007104..3007478 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CFA70_RS15480
• Protein ID: WP_161800368.1
• Coordinates: 3007478..3007720 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFA70_RS15460	3004607..3005509	+	903	WP_016155183.1	formate dehydrogenase O subunit beta	-
CFA70_RS15465	3005506..3006141	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
CFA70_RS15470	3006138..3007067	+	930	WP_003840450.1	formate dehydrogenase accessory protein FdhE	-
CFA70_RS15475	3007104..3007478	-	375	WP_003028682.1	type II toxin-antitoxin system VapC family toxin	Toxin
CFA70_RS15480	3007478..3007720	-	243	WP_161800368.1	ribbon-helix-helix domain-containing protein	Antitoxin
CFA70_RS15485	3007926..3008834	+	909	WP_088902891.1	alpha/beta hydrolase	-
CFA70_RS15495	3008985..3009926	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
CFA70_RS15500	3009971..3010408	-	438	WP_044714708.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CFA70_RS15505	3010405..3011277	-	873	WP_003028669.1	virulence factor BrkB family protein	-
CFA70_RS15510	3011271..3011870	-	600	WP_016151258.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13674.89 Da
• Isoelectric Point: 9.5622

>T78757 WP_003028682.1 NZ_CP022273:c3007478-3007104 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Nucleotide

• Length: 375 bp

>T78757 NZ_CP022273:c3007478-3007104 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9450.81 Da
• Isoelectric Point: 4.5498

>AT78757 WP_161800368.1 NZ_CP022273:c3007720-3007478 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAKTTISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT78757 NZ_CP022273:c3007720-3007478 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTAAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0J1MQ96