Detailed information of TA system TA078755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2532031..2532547
• Replicon: chromosome
• Accession: NZ_CP022273
• Organism: Citrobacter freundii strain 18-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CFA70_RS13015
• Protein ID: WP_088902793.1
• Coordinates: 2532031..2532315 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0J1MV10
• Locus tag: CFA70_RS13020
• Protein ID: WP_003839576.1
• Coordinates: 2532305..2532547 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFA70_RS13000	2527257..2528909	+	1653	WP_088902791.1	alpha,alpha-phosphotrehalase	-
CFA70_RS13005	2529318..2531456	+	2139	WP_003844922.1	anaerobic ribonucleoside-triphosphate reductase	-
CFA70_RS13010	2531563..2532027	+	465	WP_088902792.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
CFA70_RS13015	2532031..2532315	-	285	WP_088902793.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFA70_RS13020	2532305..2532547	-	243	WP_003839576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CFA70_RS13025	2532625..2534538	-	1914	WP_061548941.1	BglG family transcription antiterminator	-
CFA70_RS13030	2534560..2535300	-	741	WP_003025772.1	KDGP aldolase family protein	-
CFA70_RS13035	2535297..2536415	-	1119	WP_003025770.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
CFA70_RS13040	2536399..2537532	-	1134	WP_049002371.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10840.64 Da
• Isoelectric Point: 10.0482

>T78755 WP_088902793.1 NZ_CP022273:c2532315-2532031 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQFKKKLADVLLNARIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVTVFVVAVGK
REHSAVYLDANKRL

Nucleotide

• Length: 285 bp

>T78755 NZ_CP022273:c2532315-2532031 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACGCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAGCATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.34 Da
• Isoelectric Point: 5.0651

>AT78755 WP_003839576.1 NZ_CP022273:c2532547-2532305 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTVSDSEDTLIQTVRERLASPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT78755 NZ_CP022273:c2532547-2532305 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0J1MV10