Detailed information of TA system TA078754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2450347..2450923
• Replicon: chromosome
• Accession: NZ_CP022273
• Organism: Citrobacter freundii strain 18-1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: CFA70_RS12625
• Protein ID: WP_003837215.1
• Coordinates: 2450636..2450923 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CFA70_RS12620
• Protein ID: WP_003837217.1
• Coordinates: 2450347..2450649 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFA70_RS12605	2446859..2449009	+	2151	WP_088902764.1	pyruvate/proton symporter BtsT	-
CFA70_RS12610	2449120..2449314	+	195	WP_003830038.1	YbdD/YjiX family protein	-
CFA70_RS12615	2449327..2450283	+	957	WP_088902765.1	GTPase	-
CFA70_RS12620	2450347..2450649	-	303	WP_003837217.1	BrnA antitoxin family protein	Antitoxin
CFA70_RS12625	2450636..2450923	-	288	WP_003837215.1	BrnT family toxin	Toxin
CFA70_RS12630	2451158..2452324	+	1167	WP_088902766.1	restriction endonuclease	-
CFA70_RS12635	2452420..2454849	+	2430	WP_088902767.1	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11130.50 Da
• Isoelectric Point: 7.0746

>T78754 WP_003837215.1 NZ_CP022273:c2450923-2450636 [Citrobacter freundii]
MPMEFEWDANKAQSNLRKHGVRFEDAVLVFDDPQHLSRQDRHENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADSKERSRYEHG

Nucleotide

• Length: 288 bp

>T78754 NZ_CP022273:c2450923-2450636 [Citrobacter freundii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCACAAAGTAACCTGAGAAAACACGGTGTCCGGTTTGAGGATGCGGT
GCTGGTGTTTGACGATCCCCAACATCTTTCGCGGCAGGATCGGCATGAGAACGGTGAGTATCGCTGGCAGACCATCGGCC
TCGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCTTTGAAGTCATTCGTATCATCAGT
GCCAGAAAAGCAGACAGTAAGGAGAGGAGTCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11513.16 Da
• Isoelectric Point: 10.1293

>AT78754 WP_003837217.1 NZ_CP022273:c2450649-2450347 [Citrobacter freundii]
MNMVKHKRGSISALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEATRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT78754 NZ_CP022273:c2450649-2450347 [Citrobacter freundii]
ATGAACATGGTTAAGCATAAACGCGGCAGCATATCTGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAAGCGCTGGCGAA
TAAACCGGACGATGATATCGATTACAGTGACATTCCCGTGACCGAAGATAAGCAGTGGTCAGAGGCCACACGCGGTAAGT
TTTTCCGTCCGTTAAAAACGCAGGCCTCGGTGCGTATTGATGCCGATGTGATGGAGTGGCTGAAACGTCCAGGAAAAGGA
TATCAGACGCGCCTTAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG