Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1885951..1886571 Replicon chromosome
Accession NZ_CP022273
Organism Citrobacter freundii strain 18-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CFA70_RS09885 Protein ID WP_002892050.1
Coordinates 1886353..1886571 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag CFA70_RS09880 Protein ID WP_003021733.1
Coordinates 1885951..1886325 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CFA70_RS09870 1881097..1882290 + 1194 WP_088902653.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CFA70_RS09875 1882313..1885462 + 3150 WP_088902654.1 efflux RND transporter permease AcrB -
CFA70_RS09880 1885951..1886325 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
CFA70_RS09885 1886353..1886571 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
CFA70_RS09890 1886753..1887304 + 552 WP_044712836.1 maltose O-acetyltransferase -
CFA70_RS09895 1887421..1887891 + 471 WP_003021724.1 YlaC family protein -
CFA70_RS09900 1887969..1888109 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
CFA70_RS09905 1888111..1888371 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
CFA70_RS09910 1888560..1890113 + 1554 WP_044712838.1 EAL domain-containing protein -
CFA70_RS09915 1890165..1890518 - 354 WP_003831033.1 DUF1428 family protein -
CFA70_RS09920 1890583..1891212 - 630 WP_003835929.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T78753 WP_002892050.1 NZ_CP022273:1886353-1886571 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T78753 NZ_CP022273:1886353-1886571 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATCCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT78753 WP_003021733.1 NZ_CP022273:1885951-1886325 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT78753 NZ_CP022273:1885951-1886325 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References