Detailed information of TA system TA078744

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ygfYX/Cpta(toxin)
• Location: 3195367..3196024
• Replicon: chromosome
• Accession: NZ_CP022272
• Organism: Shewanella marisflavi strain EP1

Toxin (Protein)

• Gene name: ygfX
• Uniprot ID: -
• Locus tag: CFF01_RS14185
• Protein ID: WP_088905221.1
• Coordinates: 3195367..3195804 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: ygfY
• Uniprot ID: A0A4Y6J699
• Locus tag: CFF01_RS14190
• Protein ID: WP_033539774.1
• Coordinates: 3195785..3196024 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFF01_RS14160	3190843..3191319	-	477	WP_088905219.1	SoxR reducing system RseC family protein	-
CFF01_RS14165	3191327..3192259	-	933	WP_088905220.1	MucB/RseB C-terminal domain-containing protein	-
CFF01_RS14170	3192324..3192929	-	606	WP_033539778.1	anti-sigma factor	-
CFF01_RS14175	3192969..3193547	-	579	WP_033539777.1	RNA polymerase sigma factor RpoE	-
CFF01_RS14180	3193747..3195360	+	1614	WP_033539776.1	L-aspartate oxidase	-
CFF01_RS14185	3195367..3195804	-	438	WP_088905221.1	hypothetical protein	Toxin
CFF01_RS14190	3195785..3196024	-	240	WP_033539774.1	succinate dehydrogenase assembly factor 2	Antitoxin
CFF01_RS14195	3196102..3197010	-	909	WP_033539773.1	transcriptional activator NhaR	-
CFF01_RS14200	3197033..3197416	-	384	WP_033539772.1	hypothetical protein	-
CFF01_RS14205	3197413..3198588	-	1176	WP_088905222.1	Na+/H+ antiporter NhaA	-
CFF01_RS14210	3198897..3199748	-	852	WP_088905223.1	thymidylate synthase	-
CFF01_RS14215	3199825..3200619	-	795	WP_088905224.1	sulfite exporter TauE/SafE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (5-143)

Antitoxin

• Flavinator of succinate dehydrogenase (4-74)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 17012.99 Da
• Isoelectric Point: 8.6341

>T78744 WP_088905221.1 NZ_CP022272:c3195804-3195367 [Shewanella marisflavi]
VAGRRHKFDLVASFDQRLSLICFAAVCLSSFLVWPPISLLWFVIIKYALLSAVVGFFVYQFWILKHWSFGFWLDESGKIG
LDEKSQGAAQTENCVALYYRRVWVSPFAVLFWLCSDDNKRLVILWRDMFDDTGYRHLCRLLLRFG

Nucleotide

• Length: 438 bp

>T78744 NZ_CP022272:c3195804-3195367 [Shewanella marisflavi]
GTGGCCGGGCGGCGCCATAAGTTTGACCTTGTCGCTTCATTTGATCAGCGACTCTCGCTGATATGTTTTGCGGCCGTTTG
TCTAAGTTCATTCTTGGTCTGGCCGCCAATTTCCCTTCTCTGGTTTGTTATTATCAAATACGCCCTGCTATCGGCAGTGG
TCGGTTTCTTTGTTTATCAATTTTGGATACTCAAACACTGGTCATTTGGCTTCTGGCTGGATGAGTCCGGTAAGATTGGG
CTGGATGAAAAGTCACAAGGCGCTGCGCAAACAGAAAACTGTGTGGCTCTGTATTATCGGCGGGTGTGGGTGAGTCCTTT
TGCCGTACTCTTTTGGCTCTGTAGTGATGACAATAAGCGTTTGGTGATTCTGTGGCGGGATATGTTTGACGACACAGGCT
ATCGGCACCTGTGTCGTCTATTGCTCAGATTTGGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9192.70 Da
• Isoelectric Point: 4.7627

>AT78744 WP_033539774.1 NZ_CP022272:c3196024-3195785 [Shewanella marisflavi]
MNIARVRWACRRGMLELDVLFQPFVETQYEHLSNDDKAVFVRLLECEDPELFAWFMGHEVCPDPLLADMVVKVRGRAAP

Nucleotide

• Length: 240 bp

>AT78744 NZ_CP022272:c3196024-3195785 [Shewanella marisflavi]
ATGAACATTGCCAGAGTAAGATGGGCGTGCCGACGCGGCATGTTAGAGCTTGATGTATTGTTTCAACCCTTTGTTGAAAC
GCAATATGAGCATCTTTCTAATGATGACAAAGCTGTTTTTGTTCGTTTGTTAGAGTGTGAAGATCCCGAGTTGTTTGCCT
GGTTTATGGGTCATGAAGTGTGTCCCGATCCTCTGCTTGCCGATATGGTCGTAAAAGTTCGTGGCCGGGCGGCGCCATAA

Structures

Antitoxin

• AlphaFold DB: A0A4Y6J699