Detailed information of TA system TA078740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3887..4428
• Replicon: plasmid plA
• Accession: NZ_CP022268
• Organism: Xanthomonas citri pv. vignicola strain CFBP7112

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A220WPB1
• Locus tag: XcvCFBP7112P_RS22240
• Protein ID: WP_005917856.1
• Coordinates: 3887..4210 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: XcvCFBP7112P_RS22245
• Protein ID: WP_089142781.1
• Coordinates: 4207..4428 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XcvCFBP7112P_RS22220	304..1620	+	1317	Protein_1	AAA family ATPase	-
XcvCFBP7112P_RS22225	1910..2194	+	285	WP_003490829.1	hypothetical protein	-
XcvCFBP7112P_RS22230	2380..3567	-	1188	WP_029220895.1	zeta toxin family protein	-
XcvCFBP7112P_RS22235	3574..3861	-	288	WP_029220894.1	hypothetical protein	-
XcvCFBP7112P_RS22240	3887..4210	-	324	WP_005917856.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XcvCFBP7112P_RS22245	4207..4428	-	222	WP_089142781.1	antitoxin MazE family protein	Antitoxin
XcvCFBP7112P_RS22255	4626..5192	+	567	WP_039447693.1	recombinase family protein	-
XcvCFBP7112P_RS22265	5612..9103	+	3492	WP_089142782.1	TAL effector repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65309	65309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11513.56 Da
• Isoelectric Point: 6.2913

>T78740 WP_005917856.1 NZ_CP022268:c4210-3887 [Xanthomonas citri pv. vignicola]
MTQRGDLVTVALPGAYGKPRPALVIQSDLLAELESVLVCPITSDLRDAMFRVTVEPSLTNGLQKISQVMVDKTAPIPRAK
VSEPIGTLDAERMRAVERALMLVTGLA

Nucleotide

• Length: 324 bp

>T78740 NZ_CP022268:c4210-3887 [Xanthomonas citri pv. vignicola]
ATGACGCAGCGTGGCGACTTGGTAACGGTGGCTTTGCCGGGAGCCTACGGCAAGCCACGGCCGGCGCTAGTCATCCAGTC
CGATTTGCTTGCCGAGCTGGAAAGCGTCCTCGTATGCCCGATCACAAGCGATCTGCGCGACGCGATGTTTCGGGTCACGG
TCGAGCCGAGTCTGACCAATGGTCTACAGAAGATTTCGCAGGTCATGGTGGACAAGACAGCACCGATACCTCGCGCGAAA
GTCAGTGAGCCGATTGGCACGCTCGACGCAGAACGCATGAGGGCCGTCGAACGCGCCCTCATGCTCGTCACCGGGCTGGC
ATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8238.25 Da
• Isoelectric Point: 8.1023

>AT78740 WP_089142781.1 NZ_CP022268:c4428-4207 [Xanthomonas citri pv. vignicola]
MPRTHNPTNKVTRHRDRLRAAGYRPVQFWVHDTRSPELAAMLSRQCRSLHGDPVETDAIGFGEAAAGLIEGWE

Nucleotide

• Length: 222 bp

>AT78740 NZ_CP022268:c4428-4207 [Xanthomonas citri pv. vignicola]
ATGCCGAGAACTCACAATCCGACGAACAAGGTCACCCGTCATCGTGACCGACTGCGTGCAGCCGGGTACAGGCCAGTGCA
GTTTTGGGTGCACGATACCCGCTCGCCCGAGTTGGCTGCGATGCTCAGCCGCCAGTGTCGCAGCCTGCATGGTGATCCAG
TCGAAACCGATGCTATCGGCTTCGGTGAAGCCGCCGCTGGCCTTATCGAGGGGTGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A220WPB1