Detailed information of TA system TA078734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF-doc
• Location: 13660..14243
• Replicon: plasmid pAMT1
• Accession: NZ_CP022249
• Organism: Staphylococcus epidermidis strain AMT

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: CFE88_RS12410
• Protein ID: WP_058703189.1
• Coordinates: 13905..14243 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: CFE88_RS12405
• Protein ID: WP_088922895.1
• Coordinates: 13660..13908 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFE88_RS12370	9298..9885	+	588	WP_088922898.1	hypothetical protein	-
CFE88_RS12375	10227..10760	+	534	WP_002477877.1	DUF536 domain-containing protein	-
CFE88_RS12380	10760..11533	+	774	WP_088922897.1	hypothetical protein	-
CFE88_RS12385	11773..12039	-	267	WP_002477879.1	Txe/YoeB family addiction module toxin	-
CFE88_RS12390	12039..12290	-	252	WP_002477880.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CFE88_RS12395	12308..12514	-	207	WP_002477881.1	hypothetical protein	-
CFE88_RS12400	12766..13311	+	546	WP_088922896.1	hypothetical protein	-
CFE88_RS12405	13660..13908	+	249	WP_088922895.1	AbrB family transcriptional regulator	Antitoxin
CFE88_RS12410	13905..14243	+	339	WP_058703189.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CFE88_RS12415	14777..15154	+	378	WP_088922894.1	hypothetical protein	-
CFE88_RS12420	15452..17578	+	2127	WP_088922899.1	hypothetical protein	-
CFE88_RS12425	18551..18907	+	357	WP_088922900.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37770	37770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13106.20 Da
• Isoelectric Point: 9.6404

>T78734 WP_058703189.1 NZ_CP022249:13905-14243 [Staphylococcus epidermidis]
MSIKQFDIYYIDLDPTRGREKQKIRPCLIVNNKMTIDGTNFVWALPITNREKRYPLDIEVKTKKGLVTGVIDTLQIRALD
LNEREHNYKDELQDNLKNVVLQAIKTYLKPSS

Nucleotide

• Length: 339 bp

>T78734 NZ_CP022249:13905-14243 [Staphylococcus epidermidis]
ATGAGCATCAAACAGTTTGATATATACTATATTGACCTAGACCCCACTCGAGGTCGTGAAAAACAAAAAATCAGACCATG
CTTAATCGTAAATAATAAAATGACAATAGATGGTACAAATTTCGTTTGGGCGTTACCAATCACAAATCGCGAGAAACGTT
ATCCATTAGATATCGAAGTGAAAACTAAAAAAGGATTAGTCACAGGTGTTATAGACACGCTTCAGATACGCGCATTAGAC
CTTAATGAACGTGAACATAACTATAAAGATGAATTACAAGACAACTTAAAAAACGTCGTCTTACAAGCAATAAAGACGTA
TTTAAAACCATCATCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9175.33 Da
• Isoelectric Point: 4.6802

>AT78734 WP_088922895.1 NZ_CP022249:13660-13908 [Staphylococcus epidermidis]
MIKTSERIFKSGNSKALSLSKQTIQLANFEVGDKVEVSEINGGLFIKKKEESIEDEITNFFKNGGKYTEPEIDFGESVGR
EI

Nucleotide

• Length: 249 bp

>AT78734 NZ_CP022249:13660-13908 [Staphylococcus epidermidis]
ATGATTAAAACATCTGAACGCATTTTTAAAAGTGGAAACAGCAAAGCACTCAGTTTAAGCAAACAAACAATCCAGTTAGC
TAATTTTGAGGTGGGAGATAAGGTTGAAGTATCTGAAATCAATGGTGGTTTATTTATCAAAAAGAAAGAAGAATCGATTG
AAGATGAAATCACAAACTTTTTCAAAAATGGCGGTAAATACACCGAGCCAGAAATTGATTTTGGAGAGAGTGTAGGTCGT
GAAATATGA