Detailed information of TA system TA078730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 256315..256844
• Replicon: chromosome
• Accession: NZ_CP022247
• Organism: Staphylococcus epidermidis strain AMT

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A939SRT1
• Locus tag: CFE88_RS01400
• Protein ID: WP_002447757.1
• Coordinates: 256482..256844 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5HME6
• Locus tag: CFE88_RS01395
• Protein ID: WP_001829931.1
• Coordinates: 256315..256485 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFE88_RS01370	252139..252618	+	480	WP_002447762.1	PH domain-containing protein	-
CFE88_RS01375	252611..254116	+	1506	WP_002447761.1	PH domain-containing protein	-
CFE88_RS01380	254103..254621	+	519	WP_002447760.1	PH domain-containing protein	-
CFE88_RS01385	254660..255013	+	354	WP_002447759.1	holo-ACP synthase	-
CFE88_RS01390	255080..256228	+	1149	WP_002458690.1	alanine racemase	-
CFE88_RS01395	256315..256485	+	171	WP_001829931.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CFE88_RS01400	256482..256844	+	363	WP_002447757.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CFE88_RS01405	257190..258191	+	1002	WP_002447756.1	PP2C family protein-serine/threonine phosphatase	-
CFE88_RS01410	258291..258617	+	327	WP_001829952.1	anti-sigma factor antagonist	-
CFE88_RS01415	258619..259098	+	480	WP_002447755.1	anti-sigma B factor RsbW	-
CFE88_RS01420	259073..259843	+	771	WP_002447754.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13483.62 Da
• Isoelectric Point: 9.9522

>T78730 WP_002447757.1 NZ_CP022247:256482-256844 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSDSKMIEVDNALDISLGLNNFDHHKS

Nucleotide

• Length: 363 bp

>T78730 NZ_CP022247:256482-256844 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGATAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGATAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6289.92 Da
• Isoelectric Point: 4.1920

>AT78730 WP_001829931.1 NZ_CP022247:256315-256485 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Nucleotide

• Length: 171 bp

>AT78730 NZ_CP022247:256315-256485 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCCA
AGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0N1EF65