Detailed information of TA system TA078725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 2018045..2018561
• Replicon: chromosome
• Accession: NZ_CP022243
• Organism: Vibrio parahaemolyticus strain PB1937

Toxin (Protein)

• Gene name: relB
• Uniprot ID: U2ZZV8
• Locus tag: CFH89_RS09925
• Protein ID: WP_005464459.1
• Coordinates: 2018045..2018314 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7W4CB60
• Locus tag: CFH89_RS09930
• Protein ID: WP_005483180.1
• Coordinates: 2018307..2018561 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFH89_RS09885	2014207..2014653	-	447	Protein_1796	GNAT family N-acetyltransferase	-
CFH89_RS09890	2014832..2015242	-	411	WP_024701711.1	hypothetical protein	-
CFH89_RS09895	2015323..2016213	-	891	WP_005485669.1	hypothetical protein	-
CFH89_RS09900	2016400..2016455	-	56	Protein_1799	DUF3265 domain-containing protein	-
CFH89_RS09915	2016723..2017523	-	801	WP_159408403.1	IS3 family transposase	-
CFH89_RS09920	2017607..2017828	-	222	Protein_1801	transposase	-
CFH89_RS09925	2018045..2018314	-	270	WP_005464459.1	Txe/YoeB family addiction module toxin	Toxin
CFH89_RS09930	2018307..2018561	-	255	WP_005483180.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CFH89_RS09935	2018664..2018783	-	120	WP_078581129.1	DUF3265 domain-containing protein	-
CFH89_RS09940	2018770..2019144	-	375	WP_025787405.1	hypothetical protein	-
CFH89_RS09950	2019793..2020188	-	396	WP_017449623.1	ACT domain-containing protein	-
CFH89_RS09955	2020342..2020737	-	396	WP_114867630.1	hypothetical protein	-
CFH89_RS09960	2020837..2021757	+	921	WP_010446562.1	IS5 family transposase	-
CFH89_RS09965	2021771..2021995	-	225	WP_114867631.1	hypothetical protein	-
CFH89_RS09970	2022144..2022605	-	462	WP_025787408.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2013732..2013893	161
	-	inside	Integron	-	-	2014345..2097853	83508
	-	inside	Integrative and Conjugative Element	-	vpadF	1966183..2026639	60456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10614.96 Da
• Isoelectric Point: 7.9816

>T78725 WP_005464459.1 NZ_CP022243:c2018314-2018045 [Vibrio parahaemolyticus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDVKRSPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T78725 NZ_CP022243:c2018314-2018045 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTATTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9481.66 Da
• Isoelectric Point: 5.0623

>AT78725 WP_005483180.1 NZ_CP022243:c2018561-2018307 [Vibrio parahaemolyticus]
MRIVSFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNVEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT78725 NZ_CP022243:c2018561-2018307 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Structures

Toxin

• AlphaFold DB: U2ZZV8

Antitoxin

• AlphaFold DB: A0A7W4CB60