Detailed information of TA system TA078716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4655267..4655793
• Replicon: chromosome
• Accession: NZ_CP022229
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: DBR04_RS24620
• Protein ID: WP_000323025.1
• Coordinates: 4655267..4655554 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: DBR04_RS24625
• Protein ID: WP_000534858.1
• Coordinates: 4655554..4655793 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS24575	4650351..4650809	-	459	WP_000526135.1	IS200/IS605 family transposase	-
DBR04_RS24580	4651260..4651475	-	216	WP_000066484.1	cold shock-like protein CspB	-
DBR04_RS24585	4651776..4651988	+	213	WP_000087756.1	cold shock-like protein CspF	-
DBR04_RS24590	4652043..4652132	+	90	WP_120795389.1	hypothetical protein	-
DBR04_RS24595	4652410..4653162	-	753	WP_001047135.1	antitermination protein	-
DBR04_RS24600	4653176..4654225	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
DBR04_RS24605	4654227..4654505	-	279	WP_012304870.1	hypothetical protein	-
DBR04_RS24610	4654572..4654823	-	252	WP_000980994.1	hypothetical protein	-
DBR04_RS24615	4655040..4655195	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
DBR04_RS24620	4655267..4655554	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
DBR04_RS24625	4655554..4655793	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
DBR04_RS24630	4655818..4656123	+	306	WP_001326990.1	hypothetical protein	-
DBR04_RS24635	4656326..4656658	+	333	WP_001301033.1	protein FlxA	-
DBR04_RS24640	4657095..4658408	-	1314	WP_000589005.1	ISNCY family transposase	-
DBR04_RS24645	4658892..4659914	-	1023	WP_000915483.1	DUF4868 domain-containing protein	-
DBR04_RS24650	4659917..4660531	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4618948..4684643	65695
	-	flank	IS/Tn	-	-	4650351..4650809	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T78716 WP_000323025.1 NZ_CP022229:c4655554-4655267 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T78716 NZ_CP022229:c4655554-4655267 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT78716 WP_000534858.1 NZ_CP022229:c4655793-4655554 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT78716 NZ_CP022229:c4655793-4655554 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8