Detailed information of TA system TA078714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4474105..4474743
• Replicon: chromosome
• Accession: NZ_CP022229
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: DBR04_RS23710
• Protein ID: WP_001306887.1
• Coordinates: 4474105..4474281 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DBR04_RS23715
• Protein ID: WP_001270286.1
• Coordinates: 4474327..4474743 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS23685	4469161..4469619	+	459	WP_000526135.1	IS200/IS605 family transposase	-
DBR04_RS23690	4469727..4470938	-	1212	WP_023143671.1	BenE family transporter YdcO	-
DBR04_RS23695	4470991..4471527	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
DBR04_RS23700	4471600..4473561	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
DBR04_RS23705	4473653..4473883	-	231	WP_000494241.1	YncJ family protein	-
DBR04_RS23710	4474105..4474281	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
DBR04_RS23715	4474327..4474743	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
DBR04_RS23720	4474822..4476228	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
DBR04_RS23725	4476473..4477618	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
DBR04_RS23730	4477636..4478649	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
DBR04_RS23735	4478650..4479591	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4469161..4469619	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T78714 WP_001306887.1 NZ_CP022229:4474105-4474281 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T78714 NZ_CP022229:4474105-4474281 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT78714 WP_001270286.1 NZ_CP022229:4474327-4474743 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT78714 NZ_CP022229:4474327-4474743 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76