Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2662099..2662621 Replicon chromosome
Accession NZ_CP022229
Organism Escherichia coli strain WCHEC96200

Toxin (Protein)


Gene name relE Uniprot ID A0A829L6G8
Locus tag DBR04_RS14910 Protein ID WP_001105433.1
Coordinates 2662331..2662621 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag DBR04_RS14905 Protein ID WP_000212715.1
Coordinates 2662099..2662341 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR04_RS14880 2657815..2659188 + 1374 WP_001522402.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -
DBR04_RS14885 2659340..2659891 - 552 WP_000166270.1 ribosome-associated protein -
DBR04_RS14890 2659985..2661337 + 1353 WP_001162173.1 metalloprotease PmbA -
DBR04_RS14895 2661521..2661907 + 387 WP_001232246.1 cytochrome b562 -
DBR04_RS14905 2662099..2662341 + 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
DBR04_RS14910 2662331..2662621 + 291 WP_001105433.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DBR04_RS14915 2662622..2663086 - 465 WP_001009182.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
DBR04_RS14920 2663271..2665409 - 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
DBR04_RS14925 2665803..2667458 - 1656 WP_021523332.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11367.23 Da        Isoelectric Point: 10.0238

>T78704 WP_001105433.1 NZ_CP022229:2662331-2662621 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T78704 NZ_CP022229:2662331-2662621 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT78704 WP_000212715.1 NZ_CP022229:2662099-2662341 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT78704 NZ_CP022229:2662099-2662341 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L6G8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References