Detailed information of TA system TA078702

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2489546..2490381
• Replicon: chromosome
• Accession: NZ_CP022229
• Organism: Escherichia coli strain WCHEC96200

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J1YPH7
• Locus tag: DBR04_RS13920
• Protein ID: WP_029701676.1
• Coordinates: 2489546..2489923 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0K5N986
• Locus tag: DBR04_RS13925
• Protein ID: WP_021553056.1
• Coordinates: 2490013..2490381 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR04_RS13900	2485913..2487451	-	1539	WP_001187196.1	lysine decarboxylation/transport transcriptional activator CadC	-
DBR04_RS13905	2488200..2489042	-	843	WP_022645115.1	DUF4942 domain-containing protein	-
DBR04_RS26860	2489127..2489549	-	423	WP_001564064.1	DUF957 domain-containing protein	-
DBR04_RS13920	2489546..2489923	-	378	WP_029701676.1	TA system toxin CbtA family protein	Toxin
DBR04_RS13925	2490013..2490381	-	369	WP_021553056.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DBR04_RS13930	2490544..2490765	-	222	WP_000692345.1	DUF987 domain-containing protein	-
DBR04_RS13935	2490830..2491306	-	477	WP_021553055.1	RadC family protein	-
DBR04_RS13940	2491322..2491801	-	480	WP_001564060.1	antirestriction protein	-
DBR04_RS13945	2492067..2492885	-	819	WP_001175165.1	DUF945 domain-containing protein	-
DBR04_RS13950	2492975..2493208	-	234	WP_001278293.1	DUF905 family protein	-
DBR04_RS13955	2493214..2493891	-	678	WP_001097302.1	hypothetical protein	-
DBR04_RS13960	2494039..2494719	-	681	WP_029702103.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2478425..2524201	45776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14066.04 Da
• Isoelectric Point: 7.8045

>T78702 WP_029701676.1 NZ_CP022229:c2489923-2489546 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T78702 NZ_CP022229:c2489923-2489546 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13615.39 Da
• Isoelectric Point: 7.0264

>AT78702 WP_021553056.1 NZ_CP022229:c2490381-2490013 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT78702 NZ_CP022229:c2490381-2490013 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J1YPH7

Antitoxin

• AlphaFold DB: A0A0K5N986