Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1023580..1024240 Replicon chromosome
Accession NZ_CP022229
Organism Escherichia coli strain WCHEC96200

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag DBR04_RS06855 Protein ID WP_029701967.1
Coordinates 1023580..1023993 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag DBR04_RS06860 Protein ID WP_000354046.1
Coordinates 1023974..1024240 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR04_RS06835 1019544..1021277 - 1734 WP_029701971.1 single-stranded-DNA-specific exonuclease RecJ -
DBR04_RS06840 1021283..1021993 - 711 WP_029701969.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
DBR04_RS06845 1022017..1022913 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
DBR04_RS06850 1023025..1023546 + 522 WP_001055874.1 flavodoxin FldB -
DBR04_RS06855 1023580..1023993 - 414 WP_029701967.1 protein YgfX Toxin
DBR04_RS06860 1023974..1024240 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
DBR04_RS06865 1024483..1025463 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
DBR04_RS06870 1025540..1026199 - 660 WP_000250274.1 hemolysin III family protein -
DBR04_RS06875 1026363..1026674 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
DBR04_RS06880 1026719..1028152 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
DBR04_RS06885 1028209..1028952 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16305.25 Da        Isoelectric Point: 11.7064

>T78696 WP_029701967.1 NZ_CP022229:c1023993-1023580 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLLHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T78696 NZ_CP022229:c1023993-1023580 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGCTTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT78696 WP_000354046.1 NZ_CP022229:c1024240-1023974 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT78696 NZ_CP022229:c1024240-1023974 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAATTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References