Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 1800..2401 Replicon plasmid p1_000200
Accession NZ_CP022228
Organism Escherichia coli strain WCHEC96200

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag DBR04_RS01290 Protein ID WP_001216041.1
Coordinates 2021..2401 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DBR04_RS01285 Protein ID WP_001190712.1
Coordinates 1800..2021 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR04_RS01275 963..1214 + 252 WP_032153798.1 DNA polymerase III subunit theta -
DBR04_RS01280 1338..1727 + 390 WP_032153799.1 DNA repair protein -
DBR04_RS01285 1800..2021 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DBR04_RS01290 2021..2401 + 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DBR04_RS01295 2437..3531 - 1095 WP_087503526.1 hypothetical protein -
DBR04_RS01300 3521..4288 - 768 WP_087503525.1 hypothetical protein -
DBR04_RS01305 4295..4957 - 663 WP_060877112.1 DUF2829 domain-containing protein -
DBR04_RS01310 4972..5466 - 495 WP_000640903.1 dUTP diphosphatase -
DBR04_RS01315 5463..5939 - 477 WP_087503524.1 hypothetical protein -
DBR04_RS01320 5936..6280 - 345 WP_087503523.1 hypothetical protein -
DBR04_RS01325 6355..7383 - 1029 WP_087503522.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92538 92538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T78690 WP_001216041.1 NZ_CP022228:2021-2401 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T78690 NZ_CP022228:2021-2401 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT78690 WP_001190712.1 NZ_CP022228:1800-2021 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT78690 NZ_CP022228:1800-2021 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGAGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References