Detailed information of TA system TA078679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 2215915..2216579
• Replicon: chromosome
• Accession: NZ_CP022223
• Organism: Mycobacterium intracellulare subsp. chimaera strain SJ42

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J9WD27
• Locus tag: CE197_RS10450
• Protein ID: WP_009954498.1
• Coordinates: 2216172..2216579 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CE197_RS10445
• Protein ID: WP_009954496.1
• Coordinates: 2215915..2216175 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE197_RS10420	2212424..2212915	-	492	WP_089151656.1	DUF1707 domain-containing protein	-
CE197_RS10425	2212985..2213581	+	597	WP_089151657.1	TetR/AcrR family transcriptional regulator	-
CE197_RS10430	2213586..2214854	-	1269	WP_089151658.1	cytochrome P450	-
CE197_RS10435	2215012..2215464	+	453	WP_042911495.1	VOC family protein	-
CE197_RS10440	2215535..2215792	+	258	WP_042911496.1	hypothetical protein	-
CE197_RS10445	2215915..2216175	+	261	WP_009954496.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CE197_RS10450	2216172..2216579	+	408	WP_009954498.1	type II toxin-antitoxin system VapC family toxin	Toxin
CE197_RS10455	2216757..2217203	+	447	Protein_2036	DUF3427 domain-containing protein	-
CE197_RS10460	2217258..2218522	+	1265	WP_087139643.1	IS3 family transposase	-
CE197_RS10465	2218551..2221211	+	2661	Protein_2038	DUF3427 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14293.28 Da
• Isoelectric Point: 4.2772

>T78679 WP_009954498.1 NZ_CP022223:2216172-2216579 [Mycobacterium intracellulare subsp. chimaera]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPVDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T78679 NZ_CP022223:2216172-2216579 [Mycobacterium intracellulare subsp. chimaera]
ATGAGCAACCCGCAGGTGGCCGGAGTGCTCGACACTTCGGTGTTCATCGCGACCGAAAGTGGTCGGCCTCTCGACGCGGC
GCTGATCCCTGATCAAGTTGGCACTACGGTTGTTACCCTCGCGGAACTGCACGTTGGTGTGTTGGCCGCGTCGACTTCCG
ATATTAGAGCGCAGCGCCTCGCGACATTGGAATCCATTGCGGATATCGAAGCGCTGCCGGTCGATGACGATGCGGCCCGG
ATGTGGGCCCGGCTGCGGATCCATCTCGCCGAGACCGGTCAACGCGTCCGGATCAACGATCTGTGGATCGCGGCCATCGC
GGCGTCCCGAGGGCTACCTGTTGTCACGCAGGACGATGACTTTGCCGTCTTGGATGGTGCGGCCAATTTGACCGTTATCC
GCGTCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT78679 WP_009954496.1 NZ_CP022223:2215915-2216175 [Mycobacterium intracellulare subsp. chimaera]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT78679 NZ_CP022223:2215915-2216175 [Mycobacterium intracellulare subsp. chimaera]
ATGTCTGAAGTGGCATCTCGTGAGCTGCGCAACGATACGGCTGGGGTGCTGCGGCGGGTTCGGGATGGAGAGGACGTCAC
CATCACTGTCAGTGGCCGACCGGTCGCAGTTCTTACCGCAGTTGTCCCTCAGCGCAGGCGTTGGCTGAGCAAGACGGAGT
TGCTGTCGCGCTTGCCCCGCGTTCAAGCGGATCCGGGATTACGCGAGGACCTTCACGCACTCGCCGGCGACACTACCGAG
GAGCTGGGCCCGATCCGATGA

Structures

Toxin

• AlphaFold DB: J9WD27