Detailed information of TA system TA078677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1183567..1184129
• Replicon: chromosome
• Accession: NZ_CP022223
• Organism: Mycobacterium intracellulare subsp. chimaera strain SJ42

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A081I0A5
• Locus tag: CE197_RS05965
• Protein ID: WP_036454801.1
• Coordinates: 1183761..1184129 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A081I0A4
• Locus tag: CE197_RS05960
• Protein ID: WP_036455532.1
• Coordinates: 1183567..1183764 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE197_RS05925	1178671..1179816	-	1146	WP_192579445.1	hypothetical protein	-
CE197_RS05930	1179946..1181019	+	1074	WP_008254380.1	redox-regulated ATPase YchF	-
CE197_RS05935	1181036..1181776	+	741	WP_042910933.1	aminoglycoside 3'-phosphotransferase	-
CE197_RS05940	1181864..1182148	+	285	WP_020821851.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
CE197_RS05945	1182163..1182408	+	246	WP_042910934.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CE197_RS05950	1182444..1182995	+	552	WP_089151405.1	VOC family protein	-
CE197_RS05955	1183074..1183472	+	399	WP_036454800.1	VOC family protein	-
CE197_RS05960	1183567..1183764	+	198	WP_036455532.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CE197_RS05965	1183761..1184129	+	369	WP_036454801.1	type II toxin-antitoxin system VapC family toxin	Toxin
CE197_RS05970	1184312..1184653	+	342	WP_192579446.1	hypothetical protein	-
CE197_RS28210	1184738..1185097	+	360	WP_192579447.1	hypothetical protein	-
CE197_RS05980	1185282..1185857	+	576	WP_198345437.1	C40 family peptidase	-
CE197_RS05985	1186219..1186542	+	324	WP_009953431.1	antibiotic biosynthesis monooxygenase	-
CE197_RS05990	1186580..1187413	-	834	WP_089151408.1	SAM-dependent methyltransferase	-
CE197_RS05995	1187448..1188131	-	684	WP_089152381.1	guanylate cyclase	-
CE197_RS06000	1188278..1188487	+	210	WP_089151409.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13435.74 Da
• Isoelectric Point: 6.4862

>T78677 WP_036454801.1 NZ_CP022223:1183761-1184129 [Mycobacterium intracellulare subsp. chimaera]
MILVDTAVWIDHLHTAELRLVKLLEIDQVGCHALVIEELALGSISRREVVLDLLANLRQFPTVQHAEILHLTGQRKLYGR
GLSAVDVHLMAAVALVEGAQLWTRDKRLKAASVDAGIALFDA

Nucleotide

• Length: 369 bp

>T78677 NZ_CP022223:1183761-1184129 [Mycobacterium intracellulare subsp. chimaera]
ATGATCCTCGTCGACACCGCGGTGTGGATCGATCATCTACACACCGCGGAGTTGCGGCTAGTCAAGCTGCTGGAGATCGA
CCAAGTTGGCTGTCATGCGCTGGTCATCGAAGAGCTGGCGCTGGGGTCGATCTCGCGACGTGAGGTGGTGCTCGACCTCC
TCGCGAATCTTCGCCAGTTTCCGACCGTCCAGCACGCCGAGATCCTTCACCTCACCGGTCAACGCAAACTGTATGGACGC
GGTCTCAGTGCGGTCGACGTCCATCTCATGGCCGCCGTCGCCCTGGTTGAAGGCGCGCAGCTGTGGACGAGAGACAAGCG
GTTGAAGGCTGCGTCGGTCGATGCGGGCATCGCACTCTTCGATGCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6871.88 Da
• Isoelectric Point: 11.6072

>AT78677 WP_036455532.1 NZ_CP022223:1183567-1183764 [Mycobacterium intracellulare subsp. chimaera]
MRTTVTIDDDLLAKAAELTGVRENVALLREGLQTLIRVESARRLAALGGTDAGAVAAPRRRRGTG

Nucleotide

• Length: 198 bp

>AT78677 NZ_CP022223:1183567-1183764 [Mycobacterium intracellulare subsp. chimaera]
ATGCGGACCACAGTCACGATCGACGACGACCTGTTGGCCAAGGCTGCTGAGTTGACCGGCGTGCGCGAGAACGTCGCGTT
GCTCCGCGAGGGATTGCAGACGCTGATCAGGGTGGAAAGCGCTCGCCGACTTGCCGCGCTCGGCGGGACGGATGCCGGGG
CGGTGGCCGCTCCCCGGCGCCGCCGTGGGACTGGATGA

Structures

Toxin

• AlphaFold DB: A0A081I0A5

Antitoxin

• AlphaFold DB: A0A081I0A4