Detailed information of TA system TA078676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 1181864..1182408
• Replicon: chromosome
• Accession: NZ_CP022223
• Organism: Mycobacterium intracellulare subsp. chimaera strain SJ42

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A854HUL3
• Locus tag: CE197_RS05945
• Protein ID: WP_042910934.1
• Coordinates: 1182163..1182408 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S4Z4W6
• Locus tag: CE197_RS05940
• Protein ID: WP_020821851.1
• Coordinates: 1181864..1182148 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE197_RS05915	1176938..1177537	-	600	WP_009953444.1	lipid droplet-associated protein	-
CE197_RS05920	1177627..1178625	+	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CE197_RS05925	1178671..1179816	-	1146	WP_192579445.1	hypothetical protein	-
CE197_RS05930	1179946..1181019	+	1074	WP_008254380.1	redox-regulated ATPase YchF	-
CE197_RS05935	1181036..1181776	+	741	WP_042910933.1	aminoglycoside 3'-phosphotransferase	-
CE197_RS05940	1181864..1182148	+	285	WP_020821851.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CE197_RS05945	1182163..1182408	+	246	WP_042910934.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CE197_RS05950	1182444..1182995	+	552	WP_089151405.1	VOC family protein	-
CE197_RS05955	1183074..1183472	+	399	WP_036454800.1	VOC family protein	-
CE197_RS05960	1183567..1183764	+	198	WP_036455532.1	type II toxin-antitoxin system VapB family antitoxin	-
CE197_RS05965	1183761..1184129	+	369	WP_036454801.1	type II toxin-antitoxin system VapC family toxin	-
CE197_RS05970	1184312..1184653	+	342	WP_192579446.1	hypothetical protein	-
CE197_RS28210	1184738..1185097	+	360	WP_192579447.1	hypothetical protein	-
CE197_RS05980	1185282..1185857	+	576	WP_198345437.1	C40 family peptidase	-
CE197_RS05985	1186219..1186542	+	324	WP_009953431.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (29-80)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9380.95 Da
• Isoelectric Point: 11.2612

>T78676 WP_042910934.1 NZ_CP022223:1182163-1182408 [Mycobacterium intracellulare subsp. chimaera]
MTAAAKRALTHLLPESVAIACWEFIRGPLAENPQRVGKPLRGQLEGRYSARRGEFRVIYRIFDERVVVPVIHIAHRRDVY
R

Nucleotide

• Length: 246 bp

>T78676 NZ_CP022223:1182163-1182408 [Mycobacterium intracellulare subsp. chimaera]
ATGACGGCAGCTGCGAAGAGGGCGCTCACTCATTTGTTGCCCGAGTCGGTGGCCATTGCTTGCTGGGAGTTCATTCGGGG
TCCGCTCGCGGAGAACCCGCAGCGGGTGGGCAAGCCGCTTCGCGGCCAGTTGGAAGGGCGATACTCGGCGCGCAGGGGCG
AGTTTCGCGTCATTTACCGGATCTTCGACGAACGGGTGGTGGTCCCCGTCATCCACATCGCGCATCGCCGCGACGTTTAC
CGGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10513.88 Da
• Isoelectric Point: 4.3705

>AT78676 WP_020821851.1 NZ_CP022223:1181864-1182148 [Mycobacterium intracellulare subsp. chimaera]
MSTLPLAEVRANLSKLVDEAVRTHERIEVTRQGRRAAVILSADDFDSIMETLAILSDQDLMREVRAAESEAEDGALYTLD
QVTEEMRAVGRLPR

Nucleotide

• Length: 285 bp

>AT78676 NZ_CP022223:1181864-1182148 [Mycobacterium intracellulare subsp. chimaera]
ATGTCGACGCTTCCGCTGGCCGAGGTCCGAGCCAACCTTTCCAAGCTGGTCGACGAGGCCGTCCGGACCCATGAGCGCAT
CGAGGTCACCAGGCAAGGTCGCCGCGCGGCGGTGATTCTCAGCGCGGATGACTTCGATTCGATTATGGAAACTCTGGCCA
TCCTCAGCGATCAAGACTTGATGCGCGAAGTCCGAGCGGCAGAGTCAGAGGCAGAGGACGGCGCGCTCTACACACTCGAT
CAGGTGACCGAGGAGATGCGTGCTGTCGGCCGGCTCCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A854HUL3

Antitoxin

• AlphaFold DB: S4Z4W6