Detailed information of TA system TA078666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 757129..757721
• Replicon: plasmid p53363
• Accession: NZ_CP022220
• Organism: Bradyrhizobium guangxiense strain CCBAU 53363

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: X268_RS38080
• Protein ID: WP_128929956.1
• Coordinates: 757533..757721 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: X268_RS38075
• Protein ID: WP_128929955.1
• Coordinates: 757129..757527 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X268_RS38055	752408..752728	+	321	WP_128929952.1	ferredoxin family protein	-
X268_RS38060	753007..754230	-	1224	WP_128929953.1	type II toxin-antitoxin system HipA family toxin	-
X268_RS38065	754223..754801	-	579	WP_128929954.1	helix-turn-helix domain-containing protein	-
X268_RS38070	755279..756475	+	1197	Protein_689	Fic family protein	-
X268_RS38075	757129..757527	-	399	WP_128929955.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
X268_RS38080	757533..757721	-	189	WP_128929956.1	type II toxin-antitoxin system HicA family toxin	Toxin
X268_RS38085	757797..758387	-	591	WP_128930143.1	UPF0149 family protein	-
X268_RS38090	758420..759979	-	1560	WP_128929539.1	IS66 family transposase	-
X268_RS38095	760030..760384	-	355	Protein_694	IS66 family insertion sequence element accessory protein TnpB	-
X268_RS38100	760381..760854	-	474	WP_128929538.1	transposase	-
X268_RS38105	761030..761404	-	375	WP_188637391.1	hypothetical protein	-
X268_RS38110	761473..761802	+	330	WP_128930197.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / gmd	1..979173	979173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7077.32 Da
• Isoelectric Point: 11.2092

>T78666 WP_128929956.1 NZ_CP022220:c757721-757533 [Bradyrhizobium guangxiense]
MKSREIISILQGDGWFEVGRKGSHVQFKHPFKKGRVIVPHPERDVPLGTLKSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T78666 NZ_CP022220:c757721-757533 [Bradyrhizobium guangxiense]
ATGAAAAGCCGGGAAATTATCTCGATCCTTCAAGGGGATGGATGGTTTGAGGTGGGCCGGAAAGGCAGCCATGTGCAGTT
CAAACATCCATTCAAGAAGGGTCGCGTAATTGTTCCGCACCCCGAACGGGATGTCCCACTCGGGACACTGAAGAGTATTG
AAAAGCAGTCAGGTCTCAAACTGAGGTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14163.96 Da
• Isoelectric Point: 4.3695

>AT78666 WP_128929955.1 NZ_CP022220:c757527-757129 [Bradyrhizobium guangxiense]
MRNYIGLIHKDADSDFGVSFPDFPGVITAGKSLDDARAMAEEALTLHIEGLAEDGEAIPEPSTLEDVMADPDHRTGVAIL
VSVKPEQPKAIRVNVTLPEDVLNQIDKYAEAHGFTRSGLLTQAAKRLIGEAA

Nucleotide

• Length: 399 bp

>AT78666 NZ_CP022220:c757527-757129 [Bradyrhizobium guangxiense]
ATGCGTAACTATATTGGTCTTATCCATAAGGATGCCGACAGCGATTTCGGCGTCTCATTTCCAGACTTCCCCGGAGTGAT
CACGGCAGGCAAAAGCCTTGATGACGCTCGTGCTATGGCAGAGGAAGCCCTCACCCTTCATATCGAGGGTCTTGCTGAAG
ATGGAGAAGCCATTCCGGAGCCCTCTACTCTAGAGGATGTTATGGCAGATCCTGATCATCGAACTGGGGTAGCTATTTTG
GTGTCGGTCAAACCCGAACAACCAAAGGCTATACGTGTGAATGTCACCCTTCCTGAGGACGTTCTGAATCAGATCGACAA
ATATGCTGAAGCGCACGGCTTTACGAGATCGGGCCTCCTAACTCAGGCCGCGAAGAGACTCATAGGAGAAGCAGCCTAA