Detailed information of TA system TA078654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 12370..12923
• Replicon: plasmid unnamed2
• Accession: NZ_CP022211
• Organism: Burkholderia gladioli pv. gladioli strain FDAARGOS_188

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: A8H28_RS17010
• Protein ID: WP_080742290.1
• Coordinates: 12630..12923 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A808W488
• Locus tag: A8H28_RS17005
• Protein ID: WP_042286915.1
• Coordinates: 12370..12642 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8H28_RS16985	8090..8623	-	534	WP_042286924.1	PAAR domain-containing protein	-
A8H28_RS16990	8660..9319	-	660	WP_052409305.1	hypothetical protein	-
A8H28_RS39005	9438..9740	-	303	WP_042286921.1	hypothetical protein	-
A8H28_RS39010	9737..10237	-	501	WP_144417590.1	hypothetical protein	-
A8H28_RS16995	10312..10620	+	309	WP_042286918.1	MobB mobilization protein	-
A8H28_RS17000	10617..12290	+	1674	WP_063769171.1	relaxase/mobilization nuclease domain-containing protein	-
A8H28_RS17005	12370..12642	+	273	WP_042286915.1	CopG family ribbon-helix-helix protein	Antitoxin
A8H28_RS17010	12630..12923	+	294	WP_080742290.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8H28_RS17015	12920..13240	+	321	WP_042286911.1	hypothetical protein	-
A8H28_RS17020	13513..13692	+	180	WP_042286906.1	hypothetical protein	-
A8H28_RS17025	13682..13996	+	315	WP_042286903.1	hypothetical protein	-
A8H28_RS17030	14030..14539	-	510	WP_042286898.1	hypothetical protein	-
A8H28_RS17035	14551..16722	-	2172	WP_042286894.1	DotA/TraY family protein	-
A8H28_RS17040	16719..17327	-	609	WP_042286891.1	hypothetical protein	-
A8H28_RS39855	17285..17569	-	285	WP_146775969.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..26710	26710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11000.51 Da
• Isoelectric Point: 8.0198

>T78654 WP_080742290.1 NZ_CP022211:12630-12923 [Burkholderia gladioli pv. gladioli]
VPHVIITPRAGQGLERCRRFLADKSETAVRRAAQAIEQRFLLLETTPAVGRPFPENPAWRELAIPFGDSGYVALYRYEPA
DDAVYVLAFRHQREAGY

Nucleotide

• Length: 294 bp

>T78654 NZ_CP022211:12630-12923 [Burkholderia gladioli pv. gladioli]
GTGCCACACGTAATTATCACGCCTCGCGCCGGTCAAGGATTGGAGCGCTGCCGGCGCTTTCTTGCCGACAAAAGCGAGAC
AGCCGTTCGGCGGGCGGCGCAGGCCATCGAGCAGCGATTTCTATTGCTGGAAACGACGCCGGCCGTTGGCCGCCCTTTTC
CTGAAAATCCGGCGTGGCGCGAGCTGGCCATTCCGTTCGGCGACTCTGGCTATGTCGCCTTGTATCGGTACGAACCGGCA
GATGATGCCGTCTACGTGCTTGCTTTCCGGCATCAGAGAGAGGCGGGGTATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10456.58 Da
• Isoelectric Point: 5.1678

>AT78654 WP_042286915.1 NZ_CP022211:12370-12642 [Burkholderia gladioli pv. gladioli]
MATSLKIDDELKGRVQHLASTRDRSPHWIMCEAIRQYVEREEARESFKQEAAASWAHYQETGLHLTGEEVRTWLKTWGTD
DETGLPECHT

Nucleotide

• Length: 273 bp

>AT78654 NZ_CP022211:12370-12642 [Burkholderia gladioli pv. gladioli]
ATGGCAACGTCACTCAAGATCGACGACGAGCTGAAGGGGCGCGTACAGCACCTTGCCAGCACGCGTGATCGGTCGCCGCA
CTGGATTATGTGCGAGGCAATCAGGCAGTATGTCGAGCGCGAAGAAGCGCGCGAAAGCTTCAAGCAGGAGGCTGCGGCCT
CCTGGGCTCACTATCAGGAAACCGGCCTGCATCTGACCGGGGAAGAGGTTCGCACCTGGCTGAAAACGTGGGGCACGGAC
GATGAAACGGGGCTGCCTGAGTGCCACACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A808W488