Detailed information of TA system TA078639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 33888..34444
• Replicon: plasmid pS68-2
• Accession: NZ_CP022183
• Organism: Aeromonas salmonicida strain S68

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2H9U809
• Locus tag: CE462_RS00595
• Protein ID: WP_042468953.1
• Coordinates: 34169..34444 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CE462_RS00590
• Protein ID: WP_174416604.1
• Coordinates: 33888..34169 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE462_RS00575	29375..30049	-	675	Protein_38	IS21 family transposase	-
CE462_RS00580	30084..33112	-	3029	Protein_39	Tn3 family transposase	-
CE462_RS00585	33116..33676	-	561	WP_042468955.1	recombinase family protein	-
CE462_RS00590	33888..34169	+	282	WP_174416604.1	ribbon-helix-helix domain-containing protein	Antitoxin
CE462_RS00595	34169..34444	+	276	WP_042468953.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CE462_RS00600	35231..36145	+	915	WP_042468951.1	TOMM precursor leader peptide-binding protein	-
CE462_RS00605	36147..36332	+	186	WP_088814096.1	hypothetical protein	-
CE462_RS00610	36381..37298	+	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
CE462_RS00615	37493..38524	+	1032	WP_088814095.1	IS630 family transposase	-
CE462_RS00620	38499..38693	+	195	WP_088814094.1	YcaO-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ascB / exsD / exsA / exsB / exsE / exsC / aopD / aopB / acrH / acrV / acrG / acrR / ascV / ascY / ascX / acr2 / acr1 / aopN / ascN / ascO / ascP / ascQ / ascR / ascS / ascT / ascU / sycO / aopO / ati2 / ati2 / ati1 / ascL / ascK / ascJ / ascI / ascH / ascG / ascF / ascE / ascD / ascC	1..62485	62485
	-	inside	IScluster/Tn	-	sycO / aopO	20917..45035	24118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10685.43 Da
• Isoelectric Point: 9.5291

>T78639 WP_042468953.1 NZ_CP022183:34169-34444 [Aeromonas salmonicida]
MELKWTSKALSDLARLYEFLALANKPAAARAVQALTQAPTILLTNPRIGEQLFQFEPREVRRILVGEYEVRYEILDSIIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T78639 NZ_CP022183:34169-34444 [Aeromonas salmonicida]
ATGGAACTGAAATGGACCAGCAAGGCGCTCTCTGATTTGGCGCGCCTGTATGAATTTCTGGCGCTGGCGAACAAGCCCGC
AGCGGCTCGGGCGGTGCAGGCACTGACACAAGCCCCGACCATTCTGCTAACCAACCCACGGATTGGAGAGCAACTATTTC
AGTTTGAGCCCCGCGAGGTGAGGCGGATTTTAGTTGGGGAGTACGAGGTTCGTTACGAGATCCTAGATTCAATCATCTAC
GTCCTACGGCTATGGCATACGCGAGAAAATCGTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10351.80 Da
• Isoelectric Point: 5.6624

>AT78639 WP_174416604.1 NZ_CP022183:33888-34169 [Aeromonas salmonicida]
MKGNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWLAQEEDRDRLTQEALADVDAGRVIDHLAVQAWA
DSLNTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT78639 NZ_CP022183:33888-34169 [Aeromonas salmonicida]
ATGAAGGGCAACATGACACAAGCAACAACCAAGGTGCTGACGGCACACGTACCACTACCGATGGCCGACAAAGTTGACCA
GATGGCTGCCCGCCTTGAACGCTCGCGCGGCTGGATCATGAAACAGGCTCTTTCCGCATGGCTCGCCCAGGAAGAAGATC
GTGATCGCCTGACCCAAGAGGCGCTGGCAGACGTGGATGCGGGTCGAGTTATCGATCACCTGGCGGTGCAGGCTTGGGCT
GACAGTCTCAACACAGACCAGCCGCTGTCGGTGCCGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2H9U809