Detailed information of TA system TA078636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 49679..50235
• Replicon: plasmid pS44-3
• Accession: NZ_CP022178
• Organism: Aeromonas salmonicida strain S44

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2H9U809
• Locus tag: CE463_RS01820
• Protein ID: WP_042468953.1
• Coordinates: 49960..50235 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CE463_RS01815
• Protein ID: WP_174416604.1
• Coordinates: 49679..49960 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE463_RS01800	45166..45840	-	675	Protein_58	IS21 family transposase	-
CE463_RS01805	45875..48903	-	3029	Protein_59	Tn3 family transposase	-
CE463_RS01810	48907..49467	-	561	WP_042468955.1	recombinase family protein	-
CE463_RS01815	49679..49960	+	282	WP_174416604.1	ribbon-helix-helix domain-containing protein	Antitoxin
CE463_RS01820	49960..50235	+	276	WP_042468953.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CE463_RS01825	51022..51936	+	915	WP_042468951.1	TOMM precursor leader peptide-binding protein	-
CE463_RS01830	51938..52123	+	186	WP_088814096.1	hypothetical protein	-
CE463_RS01835	52172..53089	+	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
CE463_RS01840	53284..54315	+	1032	WP_088814095.1	IS630 family transposase	-
CE463_RS01845	54290..54484	+	195	WP_088814094.1	YcaO-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ati2 / ati2 / ati1 / ascL / ascK / ascJ / ascI / ascH / ascG / ascF / ascE / ascD / ascC / ascB / exsD / exsA / exsB / exsE / exsC / aopD / aopB / acrH / acrV / acrG / acrR / ascV / ascY / ascX / acr2 / acr1 / aopN / ascN / ascO / ascP / ascQ / ascR / ascS / ascT / ascU / sycO / aopO	1..62513	62513
	-	inside	IScluster/Tn	-	sycO / aopO	36708..60826	24118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10685.43 Da
• Isoelectric Point: 9.5291

>T78636 WP_042468953.1 NZ_CP022178:49960-50235 [Aeromonas salmonicida]
MELKWTSKALSDLARLYEFLALANKPAAARAVQALTQAPTILLTNPRIGEQLFQFEPREVRRILVGEYEVRYEILDSIIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T78636 NZ_CP022178:49960-50235 [Aeromonas salmonicida]
ATGGAACTGAAATGGACCAGCAAGGCGCTCTCTGATTTGGCGCGCCTGTATGAATTTCTGGCGCTGGCGAACAAGCCCGC
AGCGGCTCGGGCGGTGCAGGCACTGACACAAGCCCCGACCATTCTGCTAACCAACCCACGGATTGGAGAGCAACTATTTC
AGTTTGAGCCCCGCGAGGTGAGGCGGATTTTAGTTGGGGAGTACGAGGTTCGTTACGAGATCCTAGATTCAATCATCTAC
GTCCTACGGCTATGGCATACGCGAGAAAATCGTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10351.80 Da
• Isoelectric Point: 5.6624

>AT78636 WP_174416604.1 NZ_CP022178:49679-49960 [Aeromonas salmonicida]
MKGNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWLAQEEDRDRLTQEALADVDAGRVIDHLAVQAWA
DSLNTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT78636 NZ_CP022178:49679-49960 [Aeromonas salmonicida]
ATGAAGGGCAACATGACACAAGCAACAACCAAGGTGCTGACGGCACACGTACCACTACCGATGGCCGACAAAGTTGACCA
GATGGCTGCCCGCCTTGAACGCTCGCGCGGCTGGATCATGAAACAGGCTCTTTCCGCATGGCTCGCCCAGGAAGAAGATC
GTGATCGCCTGACCCAAGAGGCGCTGGCAGACGTGGATGCGGGTCGAGTTATCGATCACCTGGCGGTGCAGGCTTGGGCT
GACAGTCTCAACACAGACCAGCCGCTGTCGGTGCCGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2H9U809