Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 125627..127186 Replicon plasmid pS44-1
Accession NZ_CP022176
Organism Aeromonas salmonicida strain S44

Toxin (Protein)


Gene name hipA Uniprot ID A0A806XZ83
Locus tag CE463_RS00650 Protein ID WP_047235025.1
Coordinates 125882..127186 (+) Length 435 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A189PGR7
Locus tag CE463_RS00645 Protein ID WP_011899353.1
Coordinates 125627..125878 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE463_RS00620 120773..121618 - 846 WP_073536960.1 hypothetical protein -
CE463_RS00625 121631..122581 - 951 WP_005342491.1 IS1595-like element ISKpn3 family transposase -
CE463_RS00630 122642..123295 - 654 WP_073536961.1 hypothetical protein -
CE463_RS00635 123874..124404 + 531 WP_047235027.1 hypothetical protein -
CE463_RS00640 124407..125411 + 1005 WP_047235026.1 tyrosine-type recombinase/integrase -
CE463_RS00645 125627..125878 + 252 WP_011899353.1 XRE family transcriptional regulator Antitoxin
CE463_RS00650 125882..127186 + 1305 WP_047235025.1 type II toxin-antitoxin system HipA family toxin Toxin
CE463_RS00655 127191..127685 + 495 WP_059296301.1 hypothetical protein -
CE463_RS00660 127908..129314 + 1407 WP_088821984.1 PAAR domain-containing protein -
CE463_RS00665 129343..130203 + 861 Protein_127 aminoglycoside N-acetyltransferase AAC(3)-IId -
CE463_RS00670 130216..130758 + 543 WP_000587837.1 AAA family ATPase -
CE463_RS00675 131240..131431 - 192 WP_000951934.1 hypothetical protein -
CE463_RS00680 131455..131682 + 228 WP_000248278.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IId / aph(3'')-Ib / aph(6)-Id / dfrA5 / qacE / sul1 / qnrVC6 / floR / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / mph(A) / aph(3')-Ia - 1..216870 216870
- flank IS/Tn - - 121631..122581 950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-105)

Antitoxin

HigA2-like, helix-turn-helix domain

(7-67)


Sequences

Toxin        


Download         Length: 435 a.a.        Molecular weight: 48087.16 Da        Isoelectric Point: 6.6364

>T78634 WP_047235025.1 NZ_CP022176:125882-127186 [Aeromonas salmonicida]
MRAQQLIVAMNGEVVGTLYRAGNGAMSFQYTPEWVAEPGSRPLSLSLPLGHSRIRGSEVFNFFSNLLPDSEAIIARMQAR
FHVETAHPFDLLASVGRDCVGAIQLYPPGIDIPPVTETLAEPLEDAQIEQLLEGYQVAPLGMAEDVDFRISIAGAQEKTA
LLWHQGRWQRPQGSTPTSHIFKLPIGRIVQNNIDLRDSCENEWLCLRIAKAFGFDVANADLAAFGQKRVLIVERFDRRWS
RNGEWLLRLPQEDFCQAMGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNYSIFIEP
ASSFRMTPIYDVMSAFPIFESGGIAAQKAKMAMALQGKNKQYHFAMIQPRHFISTAAHVGFSPDRATRLMHEMAADTESV
ISAVTAELPADFPEHISNAIFQGLSSQAARIMRG

Download         Length: 1305 bp

>T78634 NZ_CP022176:125882-127186 [Aeromonas salmonicida]
ATGAGAGCGCAGCAGCTGATAGTGGCAATGAACGGCGAGGTTGTGGGCACATTGTACCGAGCTGGCAACGGAGCCATGTC
ATTCCAATACACGCCGGAATGGGTCGCAGAACCAGGTTCTCGTCCGCTATCCCTCTCACTACCTCTGGGCCATAGCCGGA
TTAGAGGAAGTGAAGTCTTCAATTTTTTTAGCAACCTTCTGCCCGACTCCGAGGCGATCATTGCTAGGATGCAGGCCCGG
TTTCACGTTGAGACAGCACACCCCTTTGATCTGTTGGCTAGTGTCGGCCGAGATTGTGTTGGCGCTATCCAACTCTACCC
GCCCGGTATCGATATCCCTCCTGTCACGGAGACGCTTGCAGAGCCACTGGAAGATGCACAGATTGAGCAGTTGCTGGAAG
GCTATCAGGTGGCACCACTTGGTATGGCTGAGGATGTAGACTTTCGGATCTCGATTGCCGGTGCCCAAGAAAAGACGGCA
TTGCTTTGGCACCAAGGGCGCTGGCAGCGACCGCAGGGCAGCACCCCGACAAGTCATATCTTCAAGCTTCCCATCGGTCG
GATCGTACAAAACAACATCGACCTCCGAGACAGTTGCGAGAATGAATGGCTCTGCCTGCGGATCGCAAAGGCATTTGGCT
TTGACGTTGCCAATGCCGACTTGGCCGCATTTGGCCAAAAGCGTGTGCTGATCGTAGAGCGGTTCGATAGACGTTGGTCG
AGAAACGGTGAATGGTTGTTGCGGTTGCCTCAGGAAGATTTTTGCCAGGCGATGGGGATCGCCCCGGCTCTGAAATACGA
GTCCCATGGTGGCCCCGGCATTGCTGATGCTATGAAGTTGTTGTTGGGTTCCCGGCTAGCAGCCAAGGATAGAGAACAGT
TCTTCAAATCGCAGATCCTGTTCTGGATGTTGGCGGCGATCGATGGGCACGGCAAGAACTACAGCATCTTCATCGAGCCT
GCGTCGTCGTTCAGGATGACCCCCATCTATGATGTCATGTCAGCCTTTCCGATCTTCGAAAGCGGTGGCATTGCAGCTCA
AAAGGCCAAAATGGCGATGGCACTCCAAGGGAAAAATAAGCAGTACCACTTTGCAATGATACAGCCGCGTCACTTCATCA
GTACCGCTGCTCATGTGGGTTTTTCACCGGATAGAGCTACTCGACTTATGCACGAGATGGCTGCTGACACCGAATCGGTG
ATCTCGGCCGTAACTGCTGAGTTGCCAGCCGATTTCCCTGAGCACATCAGCAATGCCATTTTCCAAGGGCTATCCAGCCA
GGCTGCAAGGATTATGAGGGGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9290.61 Da        Isoelectric Point: 7.2047

>AT78634 WP_011899353.1 NZ_CP022176:125627-125878 [Aeromonas salmonicida]
MKVTSPASLASAIRDHRKVSKLTQKDAAALVGIKQATVSDFENRPESTKLETLFKILAALDLELHVVKRGSTLDEEQAWD
REW

Download         Length: 252 bp

>AT78634 NZ_CP022176:125627-125878 [Aeromonas salmonicida]
ATGAAGGTTACATCTCCAGCCTCTCTGGCTTCTGCTATCCGCGACCATCGCAAGGTCAGTAAACTGACGCAGAAAGATGC
TGCGGCATTGGTTGGGATTAAGCAGGCGACAGTCTCAGATTTTGAGAACCGGCCTGAGTCTACCAAGCTGGAGACGCTAT
TCAAGATCCTTGCTGCACTGGATCTGGAGCTTCATGTGGTTAAGCGGGGCTCTACCCTGGATGAGGAGCAAGCTTGGGAT
AGGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A806XZ83

Antitoxin

Source ID Structure
AlphaFold DB A0A189PGR7

References