Detailed information of TA system TA078633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4160000..4160640
• Replicon: chromosome
• Accession: NZ_CP022175
• Organism: Aeromonas salmonicida strain S121

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A807Z4U5
• Locus tag: CE456_RS21485
• Protein ID: WP_005320982.1
• Coordinates: 4160227..4160640 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A807Z5T0
• Locus tag: CE456_RS21480
• Protein ID: WP_005320985.1
• Coordinates: 4160000..4160230 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE456_RS21460	4155976..4157367	+	1392	WP_034283822.1	T3SS effector protein-tyrosine-phosphatase AopH	-
CE456_RS21465	4157440..4157841	-	402	Protein_3756	IS4 family transposase	-
CE456_RS21470	4157909..4158826	+	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
CE456_RS24920	4158946..4159119	-	174	WP_157868083.1	hypothetical protein	-
CE456_RS24690	4159243..4159632	+	390	WP_139403308.1	site-specific integrase	-
CE456_RS21475	4159545..4159931	+	387	WP_017413139.1	tyrosine-type recombinase/integrase	-
CE456_RS21480	4160000..4160230	+	231	WP_005320985.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CE456_RS21485	4160227..4160640	+	414	WP_005320982.1	PIN domain-containing protein	Toxin
CE456_RS21490	4160718..4161071	+	354	WP_017413138.1	hypothetical protein	-
CE456_RS21495	4161098..4161373	+	276	Protein_3764	hypothetical protein	-
CE456_RS21500	4161431..4162348	-	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
CE456_RS21505	4162583..4163500	-	918	WP_088814123.1	IS5-like element ISAs4 family transposase	-
CE456_RS21510	4163687..4164268	-	582	Protein_3767	subclass B2 metallo-beta-lactamase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	aopH	4148027..4163500	15473
	-	inside	IScluster/Tn	-	aopH	4148027..4165736	17709

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-42)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14935.23 Da
• Isoelectric Point: 5.7146

>T78633 WP_005320982.1 NZ_CP022175:4160227-4160640 [Aeromonas salmonicida]
VKTFMLDTCICSFIMREMPQSVLERLGAEVASGNRIVISAITYAEMRYGQIGKKASPKLGPAIDEFVRRLDGILPWDAAT
VDQTLEVRQQLAALGTPIGNNDAAIAAHALTAGCVLVTNNTREFSRVAGLLYEDWVH

Nucleotide

• Length: 414 bp

>T78633 NZ_CP022175:4160227-4160640 [Aeromonas salmonicida]
GTGAAGACGTTTATGCTCGATACCTGCATTTGTTCCTTCATCATGCGCGAAATGCCACAGTCAGTTCTTGAACGGTTGGG
CGCAGAGGTGGCGAGCGGCAATCGGATTGTGATCTCGGCAATCACCTACGCCGAAATGCGTTATGGCCAGATCGGCAAGA
AGGCTTCTCCGAAGCTGGGGCCAGCCATTGATGAATTTGTTCGTCGTCTCGATGGGATCTTGCCATGGGACGCTGCTACG
GTCGATCAGACGCTCGAAGTGCGCCAGCAGTTAGCAGCCTTGGGCACTCCGATCGGCAATAACGATGCTGCTATTGCCGC
CCACGCCCTGACGGCTGGCTGTGTACTGGTAACCAACAATACCCGCGAGTTTTCCCGAGTGGCGGGGCTGCTCTATGAAG
ACTGGGTTCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8699.88 Da
• Isoelectric Point: 4.7419

>AT78633 WP_005320985.1 NZ_CP022175:4160000-4160230 [Aeromonas salmonicida]
MRTVSIFKNGKNQAVRLPKDMEFQGVNDLEIIKNGDTITLRPARPDWFSLAECQKVDADFLQERTDVISDEGRFAL

Nucleotide

• Length: 231 bp

>AT78633 NZ_CP022175:4160000-4160230 [Aeromonas salmonicida]
ATGCGTACTGTATCAATTTTTAAAAATGGTAAAAATCAGGCGGTGAGACTGCCAAAAGACATGGAGTTTCAGGGGGTTAA
TGACCTTGAGATTATCAAAAATGGCGACACAATCACGTTGCGCCCGGCCCGCCCTGATTGGTTTTCCCTGGCGGAATGCC
AGAAGGTGGATGCAGATTTCTTGCAAGAACGTACCGATGTGATCAGCGATGAAGGACGGTTCGCCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A807Z4U5

Antitoxin

• AlphaFold DB: A0A807Z5T0