Detailed information of TA system TA078632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 14393..14949
• Replicon: plasmid pS121-3
• Accession: NZ_CP022172
• Organism: Aeromonas salmonicida strain S121

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2H9U809
• Locus tag: CE456_RS01545
• Protein ID: WP_042468953.1
• Coordinates: 14393..14668 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CE456_RS01550
• Protein ID: WP_174416604.1
• Coordinates: 14668..14949 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE456_RS01520	10145..10339	-	195	WP_088814094.1	YcaO-like family protein	-
CE456_RS01525	10314..11345	-	1032	WP_088814095.1	IS630 family transposase	-
CE456_RS01530	11540..12457	-	918	WP_076611341.1	IS5-like element ISAs4 family transposase	-
CE456_RS01535	12506..12691	-	186	WP_088814096.1	hypothetical protein	-
CE456_RS01540	12693..13607	-	915	WP_042468951.1	TOMM precursor leader peptide-binding protein	-
CE456_RS01545	14393..14668	-	276	WP_042468953.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CE456_RS01550	14668..14949	-	282	WP_174416604.1	ribbon-helix-helix domain-containing protein	Antitoxin
CE456_RS01555	15161..15721	+	561	WP_042468955.1	recombinase family protein	-
CE456_RS01560	15725..18753	+	3029	Protein_18	Tn3 family transposase	-
CE456_RS01565	18788..19462	+	675	Protein_19	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	aopO / sycO / ascU / ascT / ascT / ascS / ascR / ascQ / ascP / ascO / ascN / aopN / acr1 / acr2 / ascX / ascY / ascV / acrR / acrG / acrV / acrH / aopB / aopD / exsC / exsE / exsB / exsA / exsD / ascB / ascC / ascD / ascE / ascF / ascG / ascH / ascI / ascJ / ascK / ascK / ascL / ati1 / ati2 / ati2	1..62511	62511
	-	inside	IScluster/Tn	-	aopO / sycO	3804..27920	24116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-50)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10685.43 Da
• Isoelectric Point: 9.5291

>T78632 WP_042468953.1 NZ_CP022172:c14668-14393 [Aeromonas salmonicida]
MELKWTSKALSDLARLYEFLALANKPAAARAVQALTQAPTILLTNPRIGEQLFQFEPREVRRILVGEYEVRYEILDSIIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T78632 NZ_CP022172:c14668-14393 [Aeromonas salmonicida]
ATGGAACTGAAATGGACCAGCAAGGCGCTCTCTGATTTGGCGCGCCTGTATGAATTTCTGGCGCTGGCGAACAAGCCCGC
AGCGGCTCGGGCGGTGCAGGCACTGACACAAGCCCCGACCATTCTGCTAACCAACCCACGGATTGGAGAGCAACTATTTC
AGTTTGAGCCCCGCGAGGTGAGGCGGATTTTAGTTGGGGAGTACGAGGTTCGTTACGAGATCCTAGATTCAATCATCTAC
GTCCTACGGCTATGGCATACGCGAGAAAATCGTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10351.80 Da
• Isoelectric Point: 5.6624

>AT78632 WP_174416604.1 NZ_CP022172:c14949-14668 [Aeromonas salmonicida]
MKGNMTQATTKVLTAHVPLPMADKVDQMAARLERSRGWIMKQALSAWLAQEEDRDRLTQEALADVDAGRVIDHLAVQAWA
DSLNTDQPLSVPR

Nucleotide

• Length: 282 bp

>AT78632 NZ_CP022172:c14949-14668 [Aeromonas salmonicida]
ATGAAGGGCAACATGACACAAGCAACAACCAAGGTGCTGACGGCACACGTACCACTACCGATGGCCGACAAAGTTGACCA
GATGGCTGCCCGCCTTGAACGCTCGCGCGGCTGGATCATGAAACAGGCTCTTTCCGCATGGCTCGCCCAGGAAGAAGATC
GTGATCGCCTGACCCAAGAGGCGCTGGCAGACGTGGATGCGGGTCGAGTTATCGATCACCTGGCGGTGCAGGCTTGGGCT
GACAGTCTCAACACAGACCAGCCGCTGTCGGTGCCGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2H9U809