Detailed information of TA system TA078630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 96866..97559
• Replicon: plasmid pS121-1a
• Accession: NZ_CP022170
• Organism: Aeromonas salmonicida strain S121

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: CE456_RS00530
• Protein ID: WP_000182276.1
• Coordinates: 97203..97559 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: CE456_RS00525
• Protein ID: WP_001172026.1
• Coordinates: 96866..97201 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE456_RS00505	94451..95671	-	1221	WP_088813960.1	HD-GYP domain-containing protein	-
CE456_RS00510	95789..96169	-	381	WP_001054412.1	hypothetical protein	-
CE456_RS00515	96166..96492	-	327	WP_000091614.1	hypothetical protein	-
CE456_RS00520	96516..96851	-	336	WP_000741275.1	hypothetical protein	-
CE456_RS00525	96866..97201	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
CE456_RS00530	97203..97559	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CE456_RS00535	97751..98353	+	603	WP_010465829.1	recombinase family protein	-
CE456_RS00540	98337..101366	+	3030	WP_032432545.1	Tn3 family transposase	-
CE456_RS00545	101618..102169	+	552	WP_157669146.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrVC4 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 / aac(6')-Ib-cr / blaOXA-1 / catB3 / qacE / sul1 / tet(E) / msr(E) / mph(E) / floR	-	1..195805	195805
	-	inside	IScluster/Tn	qnrVC4 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 / aac(6')-Ib-cr / blaOXA-1 / catB3 / qacE / sul1 / tet(E) / msr(E) / mph(E) / floR	-	47371..108242	60871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T78630 WP_000182276.1 NZ_CP022170:c97559-97203 [Aeromonas salmonicida]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T78630 NZ_CP022170:c97559-97203 [Aeromonas salmonicida]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT78630 WP_001172026.1 NZ_CP022170:c97201-96866 [Aeromonas salmonicida]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT78630 NZ_CP022170:c97201-96866 [Aeromonas salmonicida]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7