Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 110706..112329 Replicon plasmid pM160133_p1
Accession NZ_CP022165
Organism Escherichia coli strain M160133

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag CE141_RS25510 Protein ID WP_000088046.1
Coordinates 110706..112031 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag CE141_RS25515 Protein ID WP_024136327.1
Coordinates 112036..112329 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE141_RS28325 107376..107675 - 300 WP_001572440.1 hypothetical protein -
CE141_RS25485 107730..107966 - 237 Protein_105 hypothetical protein -
CE141_RS25490 108420..108845 - 426 WP_001043843.1 hypothetical protein -
CE141_RS25495 109099..109914 - 816 WP_001572439.1 HNH endonuclease -
CE141_RS25500 109927..110337 - 411 WP_000985911.1 hypothetical protein -
CE141_RS25505 110439..110645 - 207 WP_000134171.1 hypothetical protein -
CE141_RS25510 110706..112031 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
CE141_RS25515 112036..112329 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
CE141_RS25525 112933..113325 - 393 WP_000341066.1 hypothetical protein -
CE141_RS25530 113698..114039 + 342 WP_001151575.1 hypothetical protein -
CE141_RS25535 114131..114322 + 192 WP_000398480.1 hypothetical protein -
CE141_RS25540 114372..114653 + 282 WP_000077926.1 hypothetical protein -
CE141_RS25545 115216..115389 - 174 Protein_116 VWA domain-containing protein -
CE141_RS25550 115455..116159 + 705 WP_001067855.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / dfrA12 / aadA2 / floR / mcr-1.1 - 1..233149 233149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T78619 WP_000088046.1 NZ_CP022165:c112031-110706 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T78619 NZ_CP022165:c112031-110706 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTAGCTGAGAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT78619 WP_024136327.1 NZ_CP022165:c112329-112036 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT78619 NZ_CP022165:c112329-112036 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References