Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3666550..3667168 Replicon chromosome
Accession NZ_CP022164
Organism Escherichia coli strain M160133

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CE141_RS18620 Protein ID WP_001291435.1
Coordinates 3666950..3667168 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CE141_RS18615 Protein ID WP_000344800.1
Coordinates 3666550..3666924 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CE141_RS18605 3661639..3662832 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CE141_RS18610 3662855..3666004 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CE141_RS18615 3666550..3666924 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CE141_RS18620 3666950..3667168 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CE141_RS18625 3667340..3667891 + 552 WP_000536391.1 maltose O-acetyltransferase -
CE141_RS18630 3668007..3668477 + 471 WP_022645280.1 YlaC family protein -
CE141_RS18635 3668641..3670191 + 1551 WP_012602175.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CE141_RS18640 3670232..3670585 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CE141_RS18650 3670964..3671275 + 312 WP_000409911.1 MGMT family protein -
CE141_RS18655 3671306..3671878 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T78614 WP_001291435.1 NZ_CP022164:3666950-3667168 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T78614 NZ_CP022164:3666950-3667168 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT78614 WP_000344800.1 NZ_CP022164:3666550-3666924 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT78614 NZ_CP022164:3666550-3666924 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References