Detailed information of TA system TA078611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2920353..2921137
• Replicon: chromosome
• Accession: NZ_CP022164
• Organism: Escherichia coli strain M160133

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: CE141_RS14820
• Protein ID: WP_000613626.1
• Coordinates: 2920643..2921137 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: CE141_RS14815
• Protein ID: WP_139352217.1
• Coordinates: 2920353..2920646 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CE141_RS14800	2915502..2916461	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
CE141_RS14810	2917034..2920219	+	3186	WP_077516769.1	ribonuclease E	-
CE141_RS14815	2920353..2920646	+	294	WP_139352217.1	DUF1778 domain-containing protein	Antitoxin
CE141_RS14820	2920643..2921137	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
CE141_RS14825	2921232..2922185	-	954	WP_001212763.1	flagellar hook-associated protein FlgL	-
CE141_RS14830	2922197..2923840	-	1644	WP_077516765.1	flagellar hook-associated protein FlgK	-
CE141_RS14835	2923906..2924847	-	942	WP_012311956.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
CE141_RS14840	2924847..2925944	-	1098	WP_000589300.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T78611 WP_000613626.1 NZ_CP022164:2920643-2921137 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T78611 NZ_CP022164:2920643-2921137 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11139.76 Da
• Isoelectric Point: 10.5423

>AT78611 WP_139352217.1 NZ_CP022164:2920353-2920646 [Escherichia coli]
MPAANNIPARREFLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT78611 NZ_CP022164:2920353-2920646 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATTTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9