Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3964640..3965190 Replicon chromosome
Accession NZ_CP022154
Organism Escherichia coli strain ABWA45

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag CES94_RS21005 Protein ID WP_001160971.1
Coordinates 3964876..3965190 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag CES94_RS21000 Protein ID WP_000125566.1
Coordinates 3964640..3964873 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CES94_RS20975 3959711..3959998 + 288 WP_000203741.1 ferredoxin-like protein FixX -
CES94_RS20980 3960056..3961387 + 1332 WP_001183198.1 MFS transporter -
CES94_RS20985 3961498..3962028 + 531 WP_000600718.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
CES94_RS20990 3962021..3963883 + 1863 WP_000377188.1 glutathione-regulated potassium-efflux system protein KefC -
CES94_RS20995 3964075..3964554 + 480 WP_001487721.1 type 3 dihydrofolate reductase -
CES94_RS21000 3964640..3964873 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
CES94_RS21005 3964876..3965190 + 315 WP_001160971.1 CcdB family protein Toxin
CES94_RS21010 3965187..3966035 - 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
CES94_RS21015 3966042..3966419 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
CES94_RS21020 3966422..3967243 - 822 WP_001065392.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
CES94_RS21025 3967240..3968229 - 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
CES94_RS21030 3968229..3969515 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T78587 WP_001160971.1 NZ_CP022154:3964876-3965190 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T78587 NZ_CP022154:3964876-3965190 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT78587 WP_000125566.1 NZ_CP022154:3964640-3964873 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT78587 NZ_CP022154:3964640-3964873 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References