Detailed information of TA system TA078563

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 871825..872525
• Replicon: chromosome
• Accession: NZ_CP022154
• Organism: Escherichia coli strain ABWA45

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CES94_RS04575
• Protein ID: WP_016240265.1
• Coordinates: 871825..872109 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A794RE17
• Locus tag: CES94_RS04580
• Protein ID: WP_016240264.1
• Coordinates: 872199..872525 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES94_RS04550	866955..867652	-	698	Protein_829	IS1 family transposase	-
CES94_RS04560	867710..869074	+	1365	Protein_830	lactate permease LctP family transporter	-
CES94_RS04570	869559..871862	+	2304	Protein_831	DUF4092 domain-containing protein	-
CES94_RS04575	871825..872109	-	285	WP_016240265.1	TA system toxin CbtA family protein	Toxin
CES94_RS04580	872199..872525	-	327	WP_016240264.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
CES94_RS04585	872544..872765	-	222	WP_016239820.1	DUF987 domain-containing protein	-
CES94_RS04590	872774..873250	-	477	WP_016243826.1	RadC family protein	-
CES94_RS04595	873266..873724	-	459	WP_000211838.1	antirestriction protein	-
CES94_RS04600	873822..874061	-	240	WP_000194654.1	DUF905 domain-containing protein	-
CES94_RS04605	874156..874860	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CES94_RS29140	875222..875398	+	177	WP_157698491.1	hypothetical protein	-
CES94_RS04615	875478..875861	+	384	WP_001201739.1	IS66 family insertion sequence hypothetical protein	-
CES94_RS04620	875858..876205	+	348	WP_000609174.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-84)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10591.11 Da
• Isoelectric Point: 5.0063

>T78563 WP_016240265.1 NZ_CP022154:c872109-871825 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTLADRYLLPTC

Nucleotide

• Length: 285 bp

>T78563 NZ_CP022154:c872109-871825 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCTTGCGGATCGTTATTTACTACCGACTTGTTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12332.00 Da
• Isoelectric Point: 7.0273

>AT78563 WP_016240264.1 NZ_CP022154:c872525-872199 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRTSITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT78563 NZ_CP022154:c872525-872199 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGACCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A794RE17