Detailed information of TA system TA078557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3619461..3620077
• Replicon: chromosome
• Accession: NZ_CP022148
• Organism: Enterobacter roggenkampii strain 704SK10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CES92_RS17645
• Protein ID: WP_084832869.1
• Coordinates: 3619706..3620077 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: CES92_RS17640
• Protein ID: WP_015569912.1
• Coordinates: 3619461..3619703 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES92_RS17610	3614880..3615350	+	471	WP_045352968.1	hypothetical protein	-
CES92_RS17615	3615439..3616038	+	600	WP_063418382.1	glucose-1-phosphatase	-
CES92_RS17620	3616032..3616913	+	882	WP_023326335.1	virulence factor BrkB family protein	-
CES92_RS17625	3616910..3617347	+	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CES92_RS17630	3617392..3618333	+	942	WP_008501835.1	fatty acid biosynthesis protein FabY	-
CES92_RS17635	3618342..3619262	-	921	WP_063418381.1	alpha/beta hydrolase	-
CES92_RS17640	3619461..3619703	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
CES92_RS17645	3619706..3620077	+	372	WP_084832869.1	type II toxin-antitoxin system VapC family toxin	Toxin
CES92_RS17650	3620117..3621046	-	930	WP_008501833.1	formate dehydrogenase accessory protein FdhE	-
CES92_RS17655	3621043..3621678	-	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
CES92_RS17660	3621675..3622577	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13700.81 Da
• Isoelectric Point: 6.3290

>T78557 WP_084832869.1 NZ_CP022148:3619706-3620077 [Enterobacter roggenkampii]
MEHMAVFDTNILIDLFNNRVEAADAIENTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T78557 NZ_CP022148:3619706-3620077 [Enterobacter roggenkampii]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GAATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTGATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATCCTAGCTACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT78557 WP_015569912.1 NZ_CP022148:3619461-3619703 [Enterobacter roggenkampii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT78557 NZ_CP022148:3619461-3619703 [Enterobacter roggenkampii]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAACCT
CAAGCAATTGCGAAATCAACCACGTGCTGAACTGCTTCGCGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3