Detailed information of TA system TA078555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2935384..2935960
• Replicon: chromosome
• Accession: NZ_CP022148
• Organism: Enterobacter roggenkampii strain 704SK10

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: CES92_RS14370
• Protein ID: WP_063418479.1
• Coordinates: 2935673..2935960 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A156JBA1
• Locus tag: CES92_RS14365
• Protein ID: WP_023293359.1
• Coordinates: 2935384..2935686 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES92_RS14340	2931491..2932036	+	546	WP_063418482.1	porin	-
CES92_RS14345	2932036..2932356	+	321	WP_008501314.1	hypothetical protein	-
CES92_RS14350	2932402..2933772	-	1371	WP_058654250.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
CES92_RS14355	2933924..2934667	+	744	WP_063418481.1	AraC family transcriptional regulator	-
CES92_RS14360	2934715..2935353	+	639	WP_063418480.1	LysE family translocator	-
CES92_RS14365	2935384..2935686	-	303	WP_023293359.1	BrnA antitoxin family protein	Antitoxin
CES92_RS14370	2935673..2935960	-	288	WP_063418479.1	BrnT family toxin	Toxin
CES92_RS14375	2936129..2937400	+	1272	WP_063418478.1	DUF445 domain-containing protein	-
CES92_RS14380	2937397..2938473	-	1077	WP_045286439.1	DUF2955 domain-containing protein	-
CES92_RS14385	2938463..2939530	-	1068	WP_088728436.1	HlyD family secretion protein	-
CES92_RS14390	2939527..2939997	-	471	WP_021240623.1	MarR family transcriptional regulator	-
CES92_RS14395	2940143..2940838	-	696	WP_063418331.1	winged helix-turn-helix domain-containing protein	-
CES92_RS14400	2940831..2940947	-	117	Protein_2776	amino acid-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11292.79 Da
• Isoelectric Point: 7.4007

>T78555 WP_063418479.1 NZ_CP022148:c2935960-2935673 [Enterobacter roggenkampii]
MPMEYEWDNNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T78555 NZ_CP022148:c2935960-2935673 [Enterobacter roggenkampii]
ATGCCAATGGAGTATGAGTGGGATAACAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAAAACGGGGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCCTTCTTGTTATTTTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11369.01 Da
• Isoelectric Point: 10.2483

>AT78555 WP_023293359.1 NZ_CP022148:c2935686-2935384 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT78555 NZ_CP022148:c2935686-2935384 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCGCCTGAGGTAAGCGCTCGTCGTGTGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGATGCGGAGATTGATTACAGCGATATTCCTGCCACGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGACCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A156JBA1