Detailed information of TA system TA078553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 1798836..1799633
• Replicon: chromosome
• Accession: NZ_CP022148
• Organism: Enterobacter roggenkampii strain 704SK10

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: CES92_RS08865
• Protein ID: WP_063417750.1
• Coordinates: 1798836..1799357 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: CES92_RS08870
• Protein ID: WP_008499829.1
• Coordinates: 1799364..1799633 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CES92_RS08835	1794419..1795108	+	690	WP_063417746.1	pyrimidine utilization protein B	-
CES92_RS08840	1795120..1795506	+	387	WP_008499822.1	pyrimidine utilization protein C	-
CES92_RS08845	1795514..1796314	+	801	WP_063417747.1	pyrimidine utilization protein D	-
CES92_RS08850	1796324..1796914	+	591	WP_063417748.1	malonic semialdehyde reductase	-
CES92_RS08855	1796924..1797418	+	495	WP_023292823.1	pyrimidine utilization flavin reductase protein F	-
CES92_RS08860	1797440..1798762	+	1323	WP_063417749.1	pyrimidine utilization transport protein G	-
CES92_RS08865	1798836..1799357	-	522	WP_063417750.1	GNAT family N-acetyltransferase	Toxin
CES92_RS08870	1799364..1799633	-	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
CES92_RS08875	1799695..1800612	-	918	WP_023292825.1	DMT family transporter	-
CES92_RS08880	1800731..1800901	-	171	WP_013097201.1	general stress protein	-
CES92_RS08885	1801293..1801889	+	597	WP_008499831.1	NAD(P)H:quinone oxidoreductase	-
CES92_RS08890	1801910..1802137	+	228	WP_008499832.1	YccJ family protein	-
CES92_RS08895	1802173..1803414	-	1242	WP_063417751.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
CES92_RS08900	1803567..1804010	+	444	WP_008499834.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19656.42 Da
• Isoelectric Point: 7.4313

>T78553 WP_063417750.1 NZ_CP022148:c1799357-1798836 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTGENSHSLF
FPTKSIERLFEQA

Nucleotide

• Length: 522 bp

>T78553 NZ_CP022148:c1799357-1798836 [Enterobacter roggenkampii]
GTGGACAATCTCACGATCGAAATGTTTTCAGAGGGAAAAGACTATGATTTCAGGGACTTCGATTGTGGTGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATTTGCTTAAAGATCGCGATG
GTCCGCGAGTCCTGGGCTATTACACGTTGTCTGGTAGTTGCTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACTTACAGGGGCTGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTAGCATCGCAGGCGGTGGGTGTACACGGAATATTTGTTGATG
CACTCAACGACCAGGCAAAACAATTTTATCTGAAGCAGGGCTTTATTCCGCTGACGGGTGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT78553 WP_008499829.1 NZ_CP022148:c1799633-1799364 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT78553 NZ_CP022148:c1799633-1799364 [Enterobacter roggenkampii]
ATGCCAGCACTGAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGGGCAGCGGAAGTCATCGAGCAGCACCGCCGTC
TCGTATTAAACGAAGCATCCTGGAATGCGGTGATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43