Detailed information of TA system TA078527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4643439..4644460
• Replicon: chromosome
• Accession: NZ_CP022129
• Organism: Methylovulum psychrotolerans strain HV10_M2

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CEK71_RS20895
• Protein ID: WP_088621192.1
• Coordinates: 4643894..4644460 (+)
• Length: 189 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: CEK71_RS20890
• Protein ID: WP_088621191.1
• Coordinates: 4643439..4643894 (+)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEK71_RS20880	4638484..4640352	+	1869	WP_088621189.1	DUF262 domain-containing protein	-
CEK71_RS20885	4640352..4643297	+	2946	WP_088621190.1	type I restriction endonuclease subunit R	-
CEK71_RS20890	4643439..4643894	+	456	WP_088621191.1	helix-turn-helix domain-containing protein	Antitoxin
CEK71_RS20895	4643894..4644460	+	567	WP_088621192.1	PIN domain-containing protein	Toxin
CEK71_RS20900	4644733..4645755	-	1023	WP_088621193.1	site-specific integrase	-
CEK71_RS20905	4646355..4646770	-	416	Protein_4127	DNA repair protein RadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 189 a.a.
• Molecular weight: 21364.72 Da
• Isoelectric Point: 5.6279

>T78527 WP_088621192.1 NZ_CP022129:4643894-4644460 [Methylovulum psychrotolerans]
MAKFSVIYDACVLYPAPLRDLLMHLALTDLFKAQWTDHIHEEWITALLRREQYDRAVLERTRDLMDTCVRDAKVFGYEDL
IEALVLPDPNDRHVLAAAIKSGADAIVTFNLKDFPSDILAKYGIEAIHPDEFIYSQIDLAPATACGAIKRQRQSLKNPPK
AKDEFLAILQKQQLPQTVSALRAYIELI

Nucleotide

• Length: 567 bp

>T78527 NZ_CP022129:4643894-4644460 [Methylovulum psychrotolerans]
ATGGCGAAATTTTCAGTTATTTATGATGCCTGTGTCTTATACCCCGCCCCTTTAAGGGATTTGTTAATGCACTTGGCCTT
AACGGATTTGTTCAAGGCACAGTGGACAGACCACATCCATGAAGAATGGATAACAGCCTTATTGAGGCGGGAACAATACG
ACAGGGCTGTTTTAGAACGGACACGCGACCTAATGGATACCTGTGTGCGTGATGCCAAGGTGTTCGGTTATGAGGACTTA
ATAGAAGCGTTAGTCTTGCCCGATCCCAATGACAGGCATGTTTTAGCCGCCGCCATTAAATCTGGCGCGGATGCGATTGT
CACTTTCAACCTAAAAGACTTCCCCAGCGATATTTTGGCAAAATACGGCATTGAGGCGATACACCCTGATGAATTCATTT
ATAGCCAGATTGACTTAGCCCCCGCCACGGCTTGCGGTGCAATCAAGCGGCAACGGCAATCATTGAAAAACCCGCCCAAA
GCCAAAGACGAGTTTTTAGCGATATTGCAAAAACAACAGCTACCCCAAACGGTATCGGCATTAAGGGCTTACATTGAGTT
AATTTAA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16731.28 Da
• Isoelectric Point: 6.4947

>AT78527 WP_088621191.1 NZ_CP022129:4643439-4643894 [Methylovulum psychrotolerans]
MPAFAIAHLPTPQETEEAKITSRALAKYAHNDRLHLKIANPPHEADDLILPGYALNLLLDILTEMSKGNAITVMPIHAEL
STQETAELLNVSRPHLVALLEQGKIPFRKVGTHRRVLAKDVFAYKQGIDADRLQALHELTAQAQELGMGYE

Nucleotide

• Length: 456 bp

>AT78527 NZ_CP022129:4643439-4643894 [Methylovulum psychrotolerans]
ATGCCAGCATTCGCCATTGCCCACCTGCCCACCCCACAAGAAACCGAAGAAGCCAAAATCACCAGCCGGGCTTTGGCAAA
GTATGCCCATAACGACAGGCTACACTTAAAAATTGCCAACCCACCGCATGAAGCGGACGATTTGATCTTGCCCGGCTACG
CGCTTAACCTGTTGCTGGACATCTTAACGGAAATGTCCAAAGGCAATGCCATAACGGTCATGCCGATACATGCCGAATTA
TCGACCCAAGAAACCGCCGAACTGTTAAACGTCAGCCGCCCGCATTTGGTCGCCTTACTCGAACAAGGTAAAATCCCTTT
TAGAAAAGTCGGCACACATCGGCGGGTGTTGGCGAAGGATGTGTTTGCTTATAAACAAGGCATTGACGCAGACCGCCTGC
AAGCCTTGCATGAGCTGACCGCCCAAGCCCAAGAGCTGGGCATGGGTTATGAATAA