Detailed information of TA system TA078522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 2535386..2535793
• Replicon: chromosome
• Accession: NZ_CP022129
• Organism: Methylovulum psychrotolerans strain HV10_M2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CEK71_RS11510
• Protein ID: WP_088621589.1
• Coordinates: 2535386..2535574 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CEK71_RS11515
• Protein ID: WP_088619520.1
• Coordinates: 2535578..2535793 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEK71_RS11490	2530957..2531916	-	960	WP_088619516.1	glycosyltransferase family 2 protein	-
CEK71_RS11495	2532165..2533187	-	1023	WP_088619517.1	glycosyl transferase family protein	-
CEK71_RS11500	2533184..2534071	-	888	WP_088619518.1	heme o synthase	-
CEK71_RS11505	2534687..2535118	+	432	WP_088619519.1	hypothetical protein	-
CEK71_RS11510	2535386..2535574	+	189	WP_088621589.1	type II toxin-antitoxin system HicA family toxin	Toxin
CEK71_RS11515	2535578..2535793	+	216	WP_088619520.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CEK71_RS11520	2536005..2536205	+	201	WP_088619521.1	hypothetical protein	-
CEK71_RS11525	2536202..2536456	+	255	WP_088619522.1	hypothetical protein	-
CEK71_RS11530	2536846..2538453	+	1608	WP_088619523.1	NAD+ synthase	-
CEK71_RS11535	2538773..2539387	+	615	WP_088619524.1	hypothetical protein	-
CEK71_RS11540	2539485..2539874	+	390	WP_198328180.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CEK71_RS11545	2539867..2540151	+	285	WP_088619525.1	helix-turn-helix domain-containing protein	-
CEK71_RS11550	2540347..2540553	+	207	WP_088619526.1	HicB family protein	-
CEK71_RS11555	2540546..2540767	+	222	WP_088619527.1	type II toxin-antitoxin system HicA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6760.82 Da
• Isoelectric Point: 10.2093

>T78522 WP_088621589.1 NZ_CP022129:2535386-2535574 [Methylovulum psychrotolerans]
MKDSEILSLLAKDDWYLVATRGSHFPFKHPVKSGRVTVPGKPSDDVAPATLNSILKQAQLKG

Nucleotide

• Length: 189 bp

>T78522 NZ_CP022129:2535386-2535574 [Methylovulum psychrotolerans]
ATGAAAGATTCAGAAATTTTGAGCCTGCTTGCCAAAGATGACTGGTATCTTGTAGCTACACGCGGTAGCCATTTCCCGTT
CAAACACCCTGTAAAATCTGGGCGCGTAACCGTTCCCGGCAAACCAAGCGATGATGTGGCTCCTGCAACGTTAAACAGTA
TTCTTAAGCAAGCGCAATTAAAGGGGTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7710.68 Da
• Isoelectric Point: 3.7917

>AT78522 WP_088619520.1 NZ_CP022129:2535578-2535793 [Methylovulum psychrotolerans]
MRYAIIIEQAEGNYSAYVPDLPGCVSTGASLDEIEQNIREAITFHLDGLREDGLPIPIPSTVVEYCELAAA

Nucleotide

• Length: 216 bp

>AT78522 NZ_CP022129:2535578-2535793 [Methylovulum psychrotolerans]
ATGCGTTACGCAATCATCATTGAACAAGCCGAAGGCAATTATTCCGCTTATGTGCCTGACCTTCCGGGGTGTGTTTCTAC
CGGGGCTTCGCTTGACGAAATAGAGCAGAACATCCGTGAGGCTATCACGTTTCATCTTGACGGTTTGCGGGAGGACGGTT
TGCCTATCCCTATCCCTTCAACTGTCGTTGAATATTGCGAGCTTGCCGCCGCTTAA