Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 113907..114643 Replicon plasmid p1605752FIB
Accession NZ_CP022125
Organism Klebsiella pneumoniae strain DHQP1605752_NV

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CEV20_RS00660 Protein ID WP_003026803.1
Coordinates 113907..114389 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CEV20_RS00665 Protein ID WP_003026799.1
Coordinates 114377..114643 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEV20_RS00640 109261..111024 - 1764 WP_004200842.1 DUF262 domain-containing protein -
CEV20_RS00645 111222..111428 - 207 Protein_104 helix-turn-helix domain-containing protein -
CEV20_RS00650 111642..112274 + 633 WP_001567369.1 hypothetical protein -
CEV20_RS00655 112303..113706 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
CEV20_RS00660 113907..114389 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CEV20_RS00665 114377..114643 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CEV20_RS00670 114888..115322 - 435 WP_001567367.1 cell envelope integrity protein TolA -
CEV20_RS00675 115369..115917 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
CEV20_RS00685 116411..116719 + 309 WP_017896554.1 hypothetical protein -
CEV20_RS00700 117618..119156 - 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
CEV20_RS00705 119205..119552 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / tet(A) - 1..187721 187721
- inside IScluster/Tn - - 112303..126696 14393


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T78501 WP_003026803.1 NZ_CP022125:c114389-113907 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T78501 NZ_CP022125:c114389-113907 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT78501 WP_003026799.1 NZ_CP022125:c114643-114377 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT78501 NZ_CP022125:c114643-114377 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References