Detailed information of TA system TA078500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 1828079..1828567
• Replicon: chromosome
• Accession: NZ_CP022123
• Organism: Fusobacterium nucleatum subsp. polymorphum strain KCOM 1275

Toxin (Protein)

• Gene name: relE
• Uniprot ID: X7RWI8
• Locus tag: CBG61_RS08950
• Protein ID: WP_032890330.1
• Coordinates: 1828304..1828567 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: X8HUL4
• Locus tag: CBG61_RS08945
• Protein ID: WP_023038160.1
• Coordinates: 1828079..1828303 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CBG61_RS08930	1823476..1824543	-	1068	WP_023038150.1	NCS2 family permease	-
CBG61_RS08935	1825045..1826520	+	1476	WP_088765252.1	cobyric acid synthase	-
CBG61_RS08940	1826646..1828001	+	1356	WP_088765253.1	sodium:alanine symporter family protein	-
CBG61_RS08945	1828079..1828303	+	225	WP_023038160.1	hypothetical protein	Antitoxin
CBG61_RS08950	1828304..1828567	+	264	WP_032890330.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CBG61_RS08955	1828633..1830090	+	1458	WP_147368669.1	HD domain-containing protein	-
CBG61_RS08960	1830112..1830651	+	540	WP_147368670.1	TlpA family protein disulfide reductase	-
CBG61_RS08965	1830773..1832155	+	1383	WP_088765255.1	putative DNA binding domain-containing protein	-
CBG61_RS08970	1832204..1832452	-	249	Protein_1719	ImmA/IrrE family metallo-endopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

• Protein of unknown function DUF6290 (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10588.47 Da
• Isoelectric Point: 9.8829

>T78500 WP_032890330.1 NZ_CP022123:1828304-1828567 [Fusobacterium nucleatum subsp. polymorphum]
MYKVYFDKKVEKDFKKLDKKVLKLILDWIENNLENIEEPRSKGKALVGNLKDYWRYRIGDYRLITKIDDGKLLIIALELK
HRKEVYE

Nucleotide

• Length: 264 bp

>T78500 NZ_CP022123:1828304-1828567 [Fusobacterium nucleatum subsp. polymorphum]
ATGTATAAGGTATATTTTGACAAAAAAGTAGAAAAAGATTTTAAAAAATTAGATAAGAAAGTTTTAAAATTAATTTTAGA
TTGGATTGAAAATAATTTAGAAAATATAGAAGAACCAAGAAGTAAAGGAAAAGCATTGGTAGGAAATTTAAAGGATTATT
GGAGATATAGAATAGGTGATTATAGATTAATTACTAAAATTGATGATGGAAAACTATTAATTATTGCATTAGAACTTAAA
CATAGGAAAGAAGTTTATGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8713.05 Da
• Isoelectric Point: 5.8834

>AT78500 WP_023038160.1 NZ_CP022123:1828079-1828303 [Fusobacterium nucleatum subsp. polymorphum]
MSNITLRLTDEEREILNNVAHLYGDKLSTAIKTILFEKIEEDYNLKIVKDFEKREKENKVELVSLSDFRKKLGV

Nucleotide

• Length: 225 bp

>AT78500 NZ_CP022123:1828079-1828303 [Fusobacterium nucleatum subsp. polymorphum]
ATGTCAAATATTACATTAAGGTTAACAGATGAAGAAAGAGAAATACTGAACAATGTAGCTCATTTATATGGTGACAAATT
ATCAACAGCAATAAAAACAATATTGTTTGAAAAAATAGAAGAAGATTATAATTTAAAGATAGTTAAAGATTTTGAAAAAA
GAGAAAAAGAAAATAAGGTGGAATTAGTTAGTTTATCTGATTTTAGAAAAAAGTTAGGTGTCTGA

Structures

Toxin

• AlphaFold DB: X7RWI8

Antitoxin

• AlphaFold DB: X8HUL4