Detailed information of TA system TA078496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 253942..254567
• Replicon: chromosome
• Accession: NZ_CP022121
• Organism: Dehalobacterium formicoaceticum strain DMC

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: CEQ75_RS01305
• Protein ID: WP_089608736.1
• Coordinates: 253942..254292 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: CEQ75_RS01310
• Protein ID: WP_089612461.1
• Coordinates: 254289..254567 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ75_RS01275	248949..249971	-	1023	WP_198306685.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	-
CEQ75_RS18220	250044..250196	-	153	WP_157677251.1	hypothetical protein	-
CEQ75_RS01280	250230..250697	-	468	WP_198306686.1	ribosomal protein S18-alanine N-acetyltransferase	-
CEQ75_RS01285	250697..251410	-	714	WP_089608733.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
CEQ75_RS01290	251391..251876	-	486	WP_198306601.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
CEQ75_RS01295	251956..253098	-	1143	WP_089608735.1	amidohydrolase	-
CEQ75_RS01300	253181..253876	-	696	WP_089612460.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
CEQ75_RS01305	253942..254292	-	351	WP_089608736.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEQ75_RS01310	254289..254567	-	279	WP_089612461.1	ribbon-helix-helix protein, CopG family	Antitoxin
CEQ75_RS01315	254675..255811	-	1137	WP_198306687.1	alanine racemase	-
CEQ75_RS01320	255817..257367	-	1551	WP_089608737.1	NAD(P)H-hydrate dehydratase	-
CEQ75_RS01325	257372..257743	-	372	WP_089608738.1	holo-ACP synthase	-
CEQ75_RS01330	257909..258076	+	168	WP_157677253.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12820.85 Da
• Isoelectric Point: 7.0156

>T78496 WP_089608736.1 NZ_CP022121:c254292-253942 [Dehalobacterium formicoaceticum]
MIVRRGEVFFAQLNPVQGSEQGGTRPVLVVQNDIGNQYSPTTIVVAITSQINKAKLPTHVELPKEKTGMEKDSVILAEQV
RTIDKSRLQQKIAVIGEDVMQRVDRALEISLGLIEV

Nucleotide

• Length: 351 bp

>T78496 NZ_CP022121:c254292-253942 [Dehalobacterium formicoaceticum]
ATGATTGTACGACGCGGGGAAGTATTTTTTGCTCAACTAAATCCCGTTCAGGGCTCGGAGCAAGGAGGCACCAGGCCTGT
TTTAGTTGTGCAAAATGATATTGGCAACCAATATAGTCCGACGACAATTGTGGTCGCAATTACCTCCCAAATCAATAAAG
CAAAACTTCCTACGCATGTGGAACTGCCCAAAGAAAAAACCGGTATGGAAAAAGATTCGGTCATTTTAGCGGAGCAGGTG
CGCACCATTGATAAAAGCCGCCTGCAACAAAAGATAGCTGTCATCGGTGAGGATGTCATGCAGCGTGTCGACCGGGCTTT
GGAGATTAGTTTGGGATTAATAGAAGTATAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10902.54 Da
• Isoelectric Point: 6.3466

>AT78496 WP_089612461.1 NZ_CP022121:c254567-254289 [Dehalobacterium formicoaceticum]
VPNLRRIMISVPDQLLQEFDGIVNDEKRNRSEFIREAMQFYIVERRRRILQEQMKKGYQEMAVINLSIACECFSMESEVF
ENTAGSRVERGK

Nucleotide

• Length: 279 bp

>AT78496 NZ_CP022121:c254567-254289 [Dehalobacterium formicoaceticum]
GTGCCCAATTTAAGAAGGATTATGATTAGCGTTCCTGATCAGCTGCTTCAAGAATTCGATGGGATTGTTAATGACGAGAA
GAGAAATAGAAGTGAATTTATCCGAGAGGCGATGCAATTCTATATTGTAGAGCGCCGCCGTCGTATTCTTCAGGAGCAAA
TGAAAAAAGGTTATCAGGAGATGGCTGTGATTAATTTATCCATTGCCTGTGAATGCTTCTCTATGGAAAGTGAAGTGTTC
GAGAACACGGCTGGAAGCCGGGTGGAGCGTGGAAAATGA