Detailed information of TA system TA078479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 98735..99366
• Replicon: chromosome
• Accession: NZ_CP022113
• Organism: Nitrospirillum amazonense CBAmc

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Y958_RS28645
• Protein ID: WP_088875347.1
• Coordinates: 98735..99142 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248K1T8
• Locus tag: Y958_RS28650
• Protein ID: WP_040849228.1
• Coordinates: 99142..99366 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y958_RS28615	94040..94390	+	351	WP_088875342.1	antibiotic biosynthesis monooxygenase	-
Y958_RS28620	94399..94983	-	585	WP_088875343.1	TetR/AcrR family transcriptional regulator	-
Y958_RS28625	95193..96293	+	1101	WP_088875344.1	HlyD family secretion protein	-
Y958_RS28630	96328..97920	+	1593	WP_088875345.1	multidrug efflux MFS transporter	-
Y958_RS28635	98005..98427	+	423	WP_088875765.1	putative toxin-antitoxin system toxin component, PIN family	-
Y958_RS28640	98424..98687	+	264	WP_088875346.1	toxin-antitoxin system HicB family antitoxin	-
Y958_RS28645	98735..99142	-	408	WP_088875347.1	type II toxin-antitoxin system VapC family toxin	Toxin
Y958_RS28650	99142..99366	-	225	WP_040849228.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Y958_RS28655	99607..100059	-	453	WP_088875348.1	carboxymuconolactone decarboxylase family protein	-
Y958_RS28660	100182..101603	+	1422	WP_088875349.1	PLP-dependent aminotransferase family protein	-
Y958_RS28665	101835..102380	+	546	WP_088875350.1	cysteine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-126)

Antitoxin

• SpoVT-AbrB domain (8-56)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15022.16 Da
• Isoelectric Point: 6.6987

>T78479 WP_088875347.1 NZ_CP022113:c99142-98735 [Nitrospirillum amazonense CBAmc]
MLRYMLDTNVCIRVLRDRPPAARARFNAAGPGGLCISTITLAELFHGAANSTQPTQNRDKVERLIARLEVLPFDDDAASH
YGDIRADLERRECLIGPYDLMIAGHARSRGLVVVTGNLGEFRRVDGLRCEDWLDA

Nucleotide

• Length: 408 bp

>T78479 NZ_CP022113:c99142-98735 [Nitrospirillum amazonense CBAmc]
ATGTTGCGCTACATGTTGGATACCAACGTCTGTATCCGGGTGCTGCGGGACAGGCCACCGGCGGCGCGCGCAAGATTCAA
CGCCGCCGGCCCCGGCGGCCTCTGCATCTCGACCATCACCTTGGCGGAGTTGTTCCACGGGGCGGCCAACTCCACGCAGC
CAACGCAAAACCGGGACAAGGTTGAGCGCCTGATCGCCCGCCTGGAGGTGCTGCCCTTCGATGACGATGCGGCATCCCAC
TATGGCGACATCCGCGCGGACCTGGAACGTCGAGAATGCCTGATTGGGCCTTATGACCTGATGATCGCCGGTCATGCACG
CAGCCGGGGCCTGGTGGTCGTGACGGGCAATCTGGGGGAGTTCCGGCGCGTGGACGGGCTGCGCTGCGAGGATTGGCTGG
ACGCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8536.59 Da
• Isoelectric Point: 4.6028

>AT78479 WP_040849228.1 NZ_CP022113:c99366-99142 [Nitrospirillum amazonense CBAmc]
MAVRTTLFQSNRSQAVRLPKDVSFPADVREVVIIREGKKRIIVPADGLWDDFFDEPAADFPDRDQPEHQAREEL

Nucleotide

• Length: 225 bp

>AT78479 NZ_CP022113:c99366-99142 [Nitrospirillum amazonense CBAmc]
ATGGCGGTCCGCACCACTCTTTTCCAATCCAATCGCAGCCAGGCCGTTCGCCTGCCCAAAGACGTCTCCTTCCCGGCGGA
CGTGCGGGAGGTGGTCATTATCCGGGAAGGCAAGAAGCGCATCATCGTACCGGCTGACGGCCTTTGGGACGACTTCTTCG
ATGAGCCCGCTGCGGATTTCCCTGACCGAGACCAACCCGAGCATCAGGCCCGGGAAGAGCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A248K1T8