Detailed information of TA system TA078478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 74746..75416
• Replicon: chromosome
• Accession: NZ_CP022112
• Organism: Nitrospirillum amazonense CBAmc

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Y958_RS22325
• Protein ID: WP_088874201.1
• Coordinates: 74746..75171 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: Y958_RS22330
• Protein ID: WP_088875162.1
• Coordinates: 75168..75416 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y958_RS22295	70478..70930	-	453	WP_088874197.1	cupin domain-containing protein	-
Y958_RS22300	70957..71904	-	948	WP_186291559.1	TlpA family protein disulfide reductase	-
Y958_RS22305	72012..73505	+	1494	WP_211098751.1	PLP-dependent aminotransferase family protein	-
Y958_RS22310	73456..73833	-	378	WP_088874198.1	hypothetical protein	-
Y958_RS22315	73866..74351	-	486	WP_088874199.1	SRPBCC family protein	-
Y958_RS22320	74367..74678	-	312	WP_088874200.1	metalloregulator ArsR/SmtB family transcription factor	-
Y958_RS22325	74746..75171	-	426	WP_088874201.1	type II toxin-antitoxin system VapC family toxin	Toxin
Y958_RS22330	75168..75416	-	249	WP_088875162.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Y958_RS22335	75565..75858	+	294	WP_088874202.1	hypothetical protein	-
Y958_RS22340	75872..77122	-	1251	WP_088874203.1	M20/M25/M40 family metallo-hydrolase	-
Y958_RS22345	77119..78345	-	1227	WP_088875163.1	allantoate amidohydrolase	-
Y958_RS22350	78363..78866	-	504	WP_088874204.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
Y958_RS22355	78863..79783	-	921	WP_088875164.1	allantoinase PuuE	-
Y958_RS22360	80038..80412	+	375	WP_088874205.1	hydroxyisourate hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15701.92 Da
• Isoelectric Point: 4.6958

>T78478 WP_088874201.1 NZ_CP022112:c75171-74746 [Nitrospirillum amazonense CBAmc]
VNYLLDTNVVSEWVKPRPDAGVIEWLAAADEDRLYLSVPTLAELRQGVQRLAPGQRRNRLDTWLRDDLPLRFDRRILAID
AAVADAWGVIVARGQATGRSISAMDAFIAATAEIHDLTLVTRNVADFGLLQQPVLNPWMAD

Nucleotide

• Length: 426 bp

>T78478 NZ_CP022112:c75171-74746 [Nitrospirillum amazonense CBAmc]
GTGAACTATCTGCTGGACACCAACGTGGTGTCGGAGTGGGTGAAGCCTCGCCCTGACGCCGGTGTCATAGAATGGCTGGC
CGCAGCAGATGAGGATCGTCTCTATCTCAGCGTCCCCACCCTGGCGGAACTGCGTCAAGGCGTGCAGCGATTGGCGCCGG
GGCAGCGCCGGAACCGACTGGATACATGGTTGCGGGATGACCTGCCCTTGCGCTTCGATCGCCGCATCCTGGCCATCGAC
GCAGCGGTGGCCGATGCCTGGGGCGTCATCGTGGCGCGCGGCCAGGCGACCGGCCGATCCATTAGCGCGATGGACGCCTT
CATTGCCGCCACGGCGGAAATTCACGACCTTACCTTGGTGACGCGCAACGTCGCCGATTTCGGGCTGCTGCAGCAGCCCG
TTTTAAATCCTTGGATGGCTGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8976.14 Da
• Isoelectric Point: 8.8327

>AT78478 WP_088875162.1 NZ_CP022112:c75416-75168 [Nitrospirillum amazonense CBAmc]
MKTWTVAEAKAKFSEVIDRAAAGTPQVISRNGRTTAVVVSAEEWERKTRRVGNLAEFFAASPLRDSGLIVERAADSAREI
DL

Nucleotide

• Length: 249 bp

>AT78478 NZ_CP022112:c75416-75168 [Nitrospirillum amazonense CBAmc]
ATGAAAACCTGGACCGTCGCTGAAGCGAAAGCCAAGTTCAGTGAGGTGATTGATCGCGCCGCCGCCGGGACCCCACAGGT
CATCTCTCGTAATGGCCGCACCACCGCCGTGGTCGTCTCGGCCGAAGAGTGGGAACGCAAGACGAGACGTGTGGGCAATC
TGGCCGAATTCTTCGCCGCGTCCCCCTTGCGTGATTCCGGCCTGATAGTGGAACGCGCGGCGGATAGCGCCCGTGAGATT
GATCTGTGA