Detailed information of TA system TA078471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2886381..2886981
• Replicon: chromosome
• Accession: NZ_CP022110
• Organism: Nitrospirillum amazonense CBAmc

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: Y958_RS13340
• Protein ID: WP_088872352.1
• Coordinates: 2886381..2886686 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: Y958_RS13345
• Protein ID: WP_088872353.1
• Coordinates: 2886667..2886981 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y958_RS13325	2882339..2882965	-	627	WP_088872349.1	arylesterase	-
Y958_RS13330	2883060..2883770	+	711	WP_088872350.1	ABC transporter ATP-binding protein	-
Y958_RS13335	2883778..2886339	+	2562	WP_088872351.1	ABC transporter permease	-
Y958_RS13340	2886381..2886686	+	306	WP_088872352.1	BrnT family toxin	Toxin
Y958_RS13345	2886667..2886981	+	315	WP_088872353.1	BrnA antitoxin family protein	Antitoxin
Y958_RS13350	2886992..2887279	+	288	WP_040850312.1	hypothetical protein	-
Y958_RS13355	2887279..2887629	+	351	WP_050898739.1	DUF86 domain-containing protein	-
Y958_RS13360	2887771..2888814	-	1044	WP_088872354.1	NAD(P)/FAD-dependent oxidoreductase	-
Y958_RS13365	2889069..2889578	+	510	WP_088872355.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
Y958_RS13370	2889575..2890603	+	1029	WP_088872356.1	phosphotransferase	-
Y958_RS13375	2890608..2891405	+	798	WP_088872357.1	nucleotidyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-92)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (40-96)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11839.37 Da
• Isoelectric Point: 5.2508

>T78471 WP_088872352.1 NZ_CP022110:2886381-2886686 [Nitrospirillum amazonense CBAmc]
MKKRFVWDPDKAASNVRKHGVTFGAAIVAFADPFALTTQDRFEDGEERWQTLGIAEGFMLLMVAHTIWELDENGEELEII
RIISARRADRQERRRYEQETR

Nucleotide

• Length: 306 bp

>T78471 NZ_CP022110:2886381-2886686 [Nitrospirillum amazonense CBAmc]
ATGAAGAAGCGCTTCGTATGGGATCCGGATAAGGCGGCCAGTAACGTCCGCAAGCACGGCGTCACCTTTGGGGCCGCCAT
CGTCGCTTTTGCGGACCCATTCGCGCTTACCACTCAAGACCGTTTCGAAGATGGCGAGGAGCGTTGGCAAACCCTCGGCA
TCGCGGAGGGATTCATGCTGCTCATGGTGGCCCACACCATCTGGGAATTGGATGAAAACGGCGAGGAGTTGGAAATCATC
CGCATTATTTCGGCCCGCCGGGCCGATCGACAGGAAAGGCGACGCTATGAACAAGAAACTCGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11896.56 Da
• Isoelectric Point: 10.0935

>AT78471 WP_088872353.1 NZ_CP022110:2886667-2886981 [Nitrospirillum amazonense CBAmc]
MNKKLVSVTIDLANPPRLSEEEKAQLKALAERPESEIDYSDIPQTTDEFWKNAVRGRFYKPTKTSTTLRIDSDVLAWLRS
QGKGYQSRINAILRREMLASLKNG

Nucleotide

• Length: 315 bp

>AT78471 NZ_CP022110:2886667-2886981 [Nitrospirillum amazonense CBAmc]
ATGAACAAGAAACTCGTTAGCGTGACCATCGACTTGGCCAATCCCCCACGTTTGAGCGAGGAGGAAAAAGCCCAGTTGAA
GGCCTTGGCCGAACGGCCCGAGAGCGAAATCGACTACAGCGACATCCCCCAGACGACAGATGAGTTCTGGAAGAACGCCG
TGCGTGGCCGCTTCTACAAGCCGACCAAGACTTCCACCACCTTACGGATCGATTCCGACGTACTGGCCTGGCTGCGGTCC
CAGGGCAAGGGCTACCAGTCCCGTATCAATGCCATTCTGCGGCGGGAGATGTTAGCTTCCCTCAAGAACGGCTGA