Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpA-higA/BrnT-HTH
Location 2110122..2110713 Replicon chromosome
Accession NZ_CP022110
Organism Nitrospirillum amazonense CBAmc

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag Y958_RS09715 Protein ID WP_088871824.1
Coordinates 2110441..2110713 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag Y958_RS09710 Protein ID WP_211098594.1
Coordinates 2110122..2110337 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y958_RS09685 2105548..2106096 + 549 WP_088871818.1 GNAT family N-acetyltransferase -
Y958_RS09690 2106312..2107244 + 933 WP_088871819.1 branched-chain amino acid ABC transporter permease LivH -
Y958_RS09695 2107285..2108562 + 1278 WP_211098592.1 high-affinity branched-chain amino acid ABC transporter permease LivM -
Y958_RS09700 2108564..2109394 + 831 WP_088871821.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
Y958_RS09705 2109406..2110119 + 714 WP_211098593.1 ATP-binding cassette domain-containing protein -
Y958_RS09710 2110122..2110337 - 216 WP_211098594.1 helix-turn-helix domain-containing protein Antitoxin
Y958_RS09715 2110441..2110713 - 273 WP_088871824.1 BrnT family toxin Toxin
Y958_RS09720 2110746..2111180 - 435 WP_088871825.1 hypothetical protein -
Y958_RS09725 2111187..2113010 - 1824 WP_088871826.1 ATP-binding cassette domain-containing protein -
Y958_RS09730 2113296..2113517 + 222 WP_040848326.1 50S ribosomal protein L31 -
Y958_RS09735 2113784..2114236 - 453 WP_088871827.1 DUF1465 family protein -
Y958_RS09740 2114590..2115066 + 477 WP_088871828.1 Hsp20 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(4-81)

Antitoxin

Cro/C1-type helix-turn-helix domain

(9-61)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10667.96 Da        Isoelectric Point: 4.9501

>T78466 WP_088871824.1 NZ_CP022110:c2110713-2110441 [Nitrospirillum amazonense CBAmc]
MFQWDKRKAAENLAKHGVRFEDACAVFRDPFAIEWPDEREDYGEERFIIIGMAEGRLLYLAYALRGDDIRLISARGAEPY
ERRRYHDENA

Download         Length: 273 bp

>T78466 NZ_CP022110:c2110713-2110441 [Nitrospirillum amazonense CBAmc]
ATGTTCCAATGGGACAAACGCAAGGCTGCTGAAAACTTGGCCAAGCACGGCGTCCGATTTGAAGATGCCTGCGCCGTCTT
CCGGGATCCGTTCGCCATCGAGTGGCCGGATGAGCGTGAGGATTATGGTGAGGAACGTTTCATCATCATCGGCATGGCGG
AGGGGCGGTTGCTTTACCTCGCCTACGCCCTCCGGGGTGATGATATCCGTCTGATCTCCGCGAGAGGAGCTGAGCCCTAT
GAGCGCCGACGATACCACGATGAAAACGCTTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8191.54 Da        Isoelectric Point: 11.4890

>AT78466 WP_211098594.1 NZ_CP022110:c2110337-2110122 [Nitrospirillum amazonense CBAmc]
MSRMRRVPQVRTMRRALGLTQEEFAARFHVPIGTLRDWEQGRKEPDATAKAYLKVIAKNPDVVQEALKRTA

Download         Length: 216 bp

>AT78466 NZ_CP022110:c2110337-2110122 [Nitrospirillum amazonense CBAmc]
ATGTCCCGTATGCGCCGGGTGCCGCAGGTGCGGACGATGCGTCGAGCGCTGGGGTTGACGCAGGAAGAGTTCGCCGCGCG
GTTCCATGTGCCCATCGGGACACTGCGCGACTGGGAACAGGGCCGGAAGGAACCGGACGCCACGGCGAAAGCCTATCTGA
AGGTGATCGCCAAGAACCCAGACGTTGTCCAGGAAGCGCTCAAGCGGACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References