Detailed information of TA system TA078465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1423185..1423819
• Replicon: chromosome
• Accession: NZ_CP022110
• Organism: Nitrospirillum amazonense CBAmc

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Y958_RS06630
• Protein ID: WP_186291310.1
• Coordinates: 1423415..1423819 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G1Y0B1
• Locus tag: Y958_RS06625
• Protein ID: WP_004273055.1
• Coordinates: 1423185..1423418 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y958_RS06620	1419853..1423113	+	3261	WP_088871373.1	error-prone DNA polymerase	-
Y958_RS06625	1423185..1423418	+	234	WP_004273055.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Y958_RS06630	1423415..1423819	+	405	WP_186291310.1	type II toxin-antitoxin system VapC family toxin	Toxin
Y958_RS06635	1423825..1424385	-	561	WP_211098557.1	MarR family winged helix-turn-helix transcriptional regulator	-
Y958_RS06640	1424668..1425465	+	798	WP_088871376.1	SDR family oxidoreductase	-
Y958_RS06645	1425470..1426942	-	1473	WP_088871377.1	phospholipase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (10-46)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14849.26 Da
• Isoelectric Point: 4.6150

>T78465 WP_186291310.1 NZ_CP022110:1423415-1423819 [Nitrospirillum amazonense CBAmc]
IVRYLLDTNIISHMIRHPDGMATEQARLVEPAALCTSIIVAAELRFGVAKKGLPALTARVESALEMLDILPFEAPADEFY
AQLRARLEGMGQPIGANDMLIAAQCLALDYILVTDNEREFARIEGLRVENWLRG

Nucleotide

• Length: 405 bp

>T78465 NZ_CP022110:1423415-1423819 [Nitrospirillum amazonense CBAmc]
ATAGTGCGCTACCTGCTGGATACCAACATCATTTCCCATATGATCCGCCATCCGGATGGGATGGCGACGGAACAGGCTCG
TTTGGTAGAGCCTGCGGCCCTTTGCACCAGCATCATCGTGGCGGCGGAGTTGCGTTTTGGCGTTGCCAAGAAAGGGCTCC
CAGCCCTGACGGCCAGGGTCGAAAGTGCCCTTGAGATGCTCGATATCTTGCCGTTCGAAGCACCTGCTGATGAATTCTAT
GCACAGTTACGGGCGCGCTTGGAGGGGATGGGGCAGCCTATCGGGGCCAATGACATGCTTATTGCCGCCCAATGTCTGGC
CCTCGACTACATTCTCGTGACTGACAACGAGCGTGAATTCGCCCGTATCGAAGGATTGCGGGTGGAAAACTGGCTGCGGG
GATAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8889.20 Da
• Isoelectric Point: 4.5098

>AT78465 WP_004273055.1 NZ_CP022110:1423185-1423418 [Nitrospirillum amazonense CBAmc]
MFPERHVKLFKNGRNQAVRIPREFELPGEDAVMRKEGNRLIIEPVKLKSFVALLDSWEPLDEDFPAIDDLPAEDVSL

Nucleotide

• Length: 234 bp

>AT78465 NZ_CP022110:1423185-1423418 [Nitrospirillum amazonense CBAmc]
ATGTTTCCGGAGCGTCACGTCAAATTATTCAAGAATGGCCGTAATCAAGCCGTTCGCATCCCCCGGGAATTCGAATTACC
GGGAGAGGATGCGGTTATGCGCAAGGAGGGGAACCGGTTGATCATTGAACCGGTCAAGCTCAAATCCTTCGTGGCATTGC
TGGATAGCTGGGAACCTTTGGACGAGGATTTTCCCGCTATTGACGATCTGCCAGCCGAAGATGTGTCGCTATAG

Structures

Antitoxin

• AlphaFold DB: G1Y0B1