Detailed information of TA system TA078463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 832623..833209
• Replicon: chromosome
• Accession: NZ_CP022110
• Organism: Nitrospirillum amazonense CBAmc

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Y958_RS03900
• Protein ID: WP_088870979.1
• Coordinates: 832862..833209 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A248JPA8
• Locus tag: Y958_RS03895
• Protein ID: WP_040850408.1
• Coordinates: 832623..832865 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y958_RS03875	827642..829462	-	1821	WP_088870977.1	M3 family oligoendopeptidase	-
Y958_RS03880	829516..830550	-	1035	WP_211098523.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
Y958_RS03885	830630..831604	-	975	WP_088870978.1	LysR family transcriptional regulator	-
Y958_RS03890	831749..832567	+	819	WP_088872462.1	dioxygenase	-
Y958_RS03895	832623..832865	+	243	WP_040850408.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Y958_RS03900	832862..833209	+	348	WP_088870979.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Y958_RS03905	833283..833546	-	264	WP_040850401.1	DUF2312 domain-containing protein	-
Y958_RS03910	833653..833961	-	309	WP_050898743.1	DUF1244 domain-containing protein	-
Y958_RS03915	833992..834816	-	825	WP_211098524.1	N-formylglutamate amidohydrolase	-
Y958_RS03920	835167..835631	+	465	WP_050898742.1	GNAT family N-acetyltransferase	-
Y958_RS03925	835703..836470	+	768	WP_088870981.1	alpha/beta fold hydrolase	-
Y958_RS03930	836507..836995	-	489	WP_088870982.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-109)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12317.34 Da
• Isoelectric Point: 8.0218

>T78463 WP_088870979.1 NZ_CP022110:832862-833209 [Nitrospirillum amazonense CBAmc]
VTEYRPEAGDLIWTDFDPRLGREQGGRRPAVVVSPAVFWDASRFAIVCPITSKIRPFGTSVVLPEGLPITGEILTSHVRS
IDTLARLIRPMGAAVPSDVLVDLRAKLGALIGLGG

Nucleotide

• Length: 348 bp

>T78463 NZ_CP022110:832862-833209 [Nitrospirillum amazonense CBAmc]
GTGACTGAATACCGGCCTGAGGCCGGGGACCTCATCTGGACGGATTTTGATCCCAGGTTGGGACGGGAACAGGGCGGCCG
CCGACCGGCCGTAGTGGTATCGCCGGCGGTGTTCTGGGATGCCAGCCGCTTCGCCATCGTCTGCCCCATCACCAGCAAGA
TTCGACCCTTCGGAACCAGCGTGGTGCTGCCGGAGGGGCTGCCGATCACGGGCGAAATACTGACCAGTCACGTGCGCAGT
ATAGATACTTTGGCGCGGCTTATCCGGCCCATGGGGGCTGCGGTTCCGTCGGATGTGCTGGTGGACTTGAGGGCGAAGCT
GGGGGCTCTCATCGGGCTTGGGGGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8739.95 Da
• Isoelectric Point: 4.7164

>AT78463 WP_040850408.1 NZ_CP022110:832623-832865 [Nitrospirillum amazonense CBAmc]
MQVQISRWGNSLGLRVPKDIATEVGLAEGDRVEVVAEHGRIVISPTRPRYQLADLLVGMTPHAMGAAFDWGDDVGREVVE

Nucleotide

• Length: 243 bp

>AT78463 NZ_CP022110:832623-832865 [Nitrospirillum amazonense CBAmc]
ATGCAGGTTCAGATTTCACGGTGGGGTAACAGCCTGGGCCTGCGGGTCCCCAAGGATATCGCCACTGAAGTGGGTCTGGC
CGAAGGCGACCGCGTGGAGGTGGTGGCCGAGCATGGCCGAATCGTCATCTCGCCGACCCGCCCGCGTTATCAGCTGGCTG
ATCTGCTCGTGGGCATGACTCCCCATGCCATGGGGGCCGCCTTCGACTGGGGTGACGATGTCGGCCGCGAGGTGGTCGAG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A248JPA8